The Development Of Retinal Vascular System In Mouse

People can easily communicate emotion in the eyes, and the outside information accepted byhumanthe brain through the visual system processing incoming and processing is completed, the first of aseries of process that occurs in the retina. Visual information form visual nerve impulses in the retina, alongthe way saw visual information to form the visual by transmitted to the visual cortex, so establish the imagein our minds. Like photographic film in a camera, the retina responsible for photographic imaging. Sensorylayer of the retina is composed of three neurons. The first neurons are visual cell layer, speciallyphotosensitive, which includes cone cells and pillar cells. Column cells in the retina far away from thefovea, while the cone cells in the central cavity at most. The second layer called bipolar cells, abouthundreds of photoreceptor cells associated with a ganglion cell through the bipolar cells, that responsiblefor liaison role. The third layer called the ganglion cell layer, which deal with the conduction specifically.The retina is a layer of transparent film, the back boundary is located around the optic nerve, the firstindustry is located in the sawtooth edge, its outside next to the choroid, situated close to vitreous body. Themacula in the posterior pole of the retina, a small concave in the central foveal macular and that isavascular area, on account of only a large number of cone cells in the area, so it is the most sensitive partsof the visual in the retina. The inner retina is a thin film and have photographic effect that is lining thevascular membrane surface. The rear of the optic nerve head. Existing the blood-retinal barrier in the retinalsimilar to that of the blood-brain barrier, the blood-retinal barrier is close connection between the capillaryendothelial cells within the eye, which lies somewhere between the blood and retinal tissue with specialpermeability. The blood-retinal barrier important relationship with retinopathy.Retinal as the fine structure and the complex anatomical structures of the nervous system, has beenthe research focus in the field of life science. In this experiment, mice were used as the object study thedevelopment of the retinal vascular system.Objective: To explore the development of vascular network and astrocytes of the mouse retina. Studythe relationship between the development of vascular network and astrocytes of the mouse retinal.Observed the development and structure of the blood-retinal barrier of mouse retina. Methods: In this experiment, we using immunohistochemical staining, gelatin ink perfusion andtransmission electron microscopy showed retinal vascular development ifferent postnatal ages of the mouseand explore structure and development of the blood-retinal barrier.Results:①R etinal vessels of mice after birth day with regard in center of nipple asymmetrical radialgrowth to see the nipples as the center in the retina surface continuously spread to the surrounding, formedthe radial network structure by capillaries interrelated, and gradually differentiate trunk arteriovenousvascular and branch, with the retina vascular network reach edge and cover the entire retina, vascularnetwork become more loose, and the retinal vascular gradually mature.②The astrocytes also began togrow from the optic disc area, in order to optic as the center to the periphery expansion, form a mesh orring contact between the cell processes and intertwining "hollow tubular" structure. To adult astrocytestypical stellate shape, the protuberance was a thin line-shaped, and more hollow tubular structure isformed,the tubular structure on the inner surface of the retina formed similar to the structure of the vascularnetwork.③A dult mice blood-retinal barrier under an optical microscope is conposed of endothelial cells,basal membrane and astrocyte foot three elements at least, and which are similar the blood-brain barrier.Electron microscope astrocytes formed first bracket, endothelial cells and pericytes constituting thevascular wall has to enter the stent internal, closely connected is formed between the endothelial cells andsecrete matrix-forming the composition of the base film, subsequent the projection of the astrocytesthinning becomes flat to form the final foot surrounding the blood vessel wall to form a completeblood-retinal barrier.Conclusions:①Mouse retinal blood vessels and astrocytes in order to the optic nerve as the centeraround gradually mature, show that the development and maturation of the retinal vascular network, thereis consistency in terms of time and space with the occurrence of retinal cells and differentiation.In addition,the retinal blood vessel formation is around P14, this is consistent with the first time in mice eyes open,indicating the formation of the vascular network has correlation with the visual system.②Blood retinalbarrier in mice the composition of vascular endothelial cells, basement membrane, cell cycle and astrocyteprocesses, the mature blood-retinal barrier endowed certain anti-infectious immunity to the retina.