Generation Of Wheat Transcription Factor Gene Transgenic Rice And Screening Of Stress Resistance Transgenic Offspring

Plant growth and productivity are greatly affected by biotic stress and abiotic stresses such asdrought, salinity, and temperature. Since the1980s，a wide range of research applications has been madein improve crop stress tolerance by genetic engineering technology，which has provides new methods tocultivation of new varieties. In signaling networks of stress induced signal to stress response genes,transcription factor play an essential role. Many transcription factor family such as MYB、MYC、ERF、bZIP and WRKY have been confirmed involved in stress response. Biological engineering research byusing transcription factors stress signals to develop stress tolerance plant has become a hotspot ofmodern molecular biology.As one of most important crops in the world, wheat is the research emphasis of genetic engineering,but the research progress is rather slow due to the complexity of genome and difficult of transformation.On the other hand, great research progress have been made in rice, another important crop, one of modelorganism in genetic engineering research. In this article, reference to the full-length cDNAover-expressing gene hunting system, wheat transcription factor full-length cDNA are transformed intorice genome on the binary vector pCUbi1390with agrobacterium-mediated transformation method..Webuild a genetically modified rice groups，and research gene function through the study of transgenic ricegroup。We screen transgenic offspring plant under drought and high salt stress to get drought resistanceand salt resistance novel rice varieties.According to traditional vector construction methods about1500required heat transcription factorfull-length Cdna,it need huge andfussy digestion and connection which waste a lot of time andenergy.While,when take advantage of gateway technology,we can greatly simplify steps of vectorconstruction and guarantee the high-efficiency of cloning at the same time.We construct the binaryvector via BP and LP reaction.Transient expression of GUS gene mediated by gene gun proveavailability of the binary vector. Then we transformed the vector into rice genome withagrobacterium-mediated transformation method.PCR was uesd to identify T2generation transgenicplant.We use20%PEG solution and50mM NaCl solution to simulated drought and high salt conditions，and nine transgenic lines are screened out. Molecular identifications prove these wheat transcriptionfactors’ expression in rice. But specific mechanism of these genes in rice need further studies to clarify.