Genetic Diversity Of Miscanthus&Triarrhena Based On ISSR And SSR Analysis

Due to the high biomass yield and low cultivation management costs, Miscanthus and Triarrhena has become one of the well-known most potential bio-energy plant. Our country, as the distribution center of Miscanthus and Triarrhena, has a large amount of germplasm resources. Among them, T.sacchariflorus, T.lutarioriparius, M.sinensis, and M.floridulus is widespread through all of the China, from the southern part, Heilongjiang province, to the northern part, Hainan province. However, our research on this is not sufficient. So, our study, using ISSR molecular marker technology and TP-M13automated fluorescent-lablled system of SSR, analyzed the genetic diversity of collected M.sinensis, including22species whose origins are known and breeding conservation of98species. The results show that:1.12pairs of ISSR primer were used to amplify223fragments, of which216(96.86%) were polymorphic. Average Nei’s gene heterozygosity is0.3018. Shannon index is0.4628, which means22species of M.sinensis have rich genetic diversity. Cluster analysis of UPGMA showed that22species could be clustered into3groups at the level:the first group was made up of T.lutarioriparius from Beijing and Hubei province, and T.sacchariflorus; the second group was made up of M.floridulus from Jiangxi, Guangdong and Hunan province; the third group was made up of T.lutarioriparius and M.sinensis from Shandong and Jiangsu province.2.14pairs of SSR primer were used to amplify205fragments, of which114(66.28%) were polymorphic. Average Nei’s gene heterozygosity is0.1120. Shannon index is0.1929, which means22species of M.sinensis have rich genetic diversity. Cluster analysis of UPGMA showed that22species could be clustered into2groups at the level:the first group was made up of T.lutarioriparius from Beijing, Shandong, Jiangsu and Hubei province; the second group was made up of M.floridulus from Guangdong Jiangxi and Hunan province.3.12pairs of ISSR primer and14pairs of SSR were used to amplify to analyze the genetic diversity of collected M.sinensis, including22species whose origins are known and breeding conservation of98species. The results show that:223fragments (100%polymorphic) were got using ISSR marker. Average Nei’s gene heterozygosity is0.3004. Shannon index is0.4650, which means120species of M.sinensis have rich genetic diversity. Cluster analysis of UPGMA showed that120species could be clustered into3groups at the level:the first group was made up of70materials including7species of M.floridulus,12 species of M.sinensis,15species of T.lutarioriparius and28species of M.floridulus etc; the second group was made up of was made up of24materials including13species of M.floridulus,8species of T.sacchariflorus,1species of M.floridulus etc; the third group was made up of was made up of26materials including8species of T.lutarioriparius,13species of T.sacchariflorus etc;14pairs of SSR primer were used to amplify205fragments, of which204(99.42%) were polymorphic. Average Nei’s gene heterozygosity is0.1119. Shannon index is0.2029, which means120species of M.sinensis have rich genetic diversity. Cluster analysis of UPGMA showed that120species could be clustered into2groups at the level:the first group was made up of111materials all of the M.sinensis, T.sacchariflorus, T.lutarioriparius included; the second group was made up of from8species M.floridulus and1species of M.sinensis.In conclusion, the genetic diversity analysis has provided the theory support from the molecular level for the collection, reservation and genetic breeding of Miscanthus and Triarrhena.