Callus Induction And Bud Proliferation Of Arnebia Euchroma(Royle) Johnst

In this research, Arnebia euchroma (Royle) Johnst as the explant, by using tissue culture techniques,formation of callus induction, callus proliferation, callus redifferentiation, inhibition of vitrification intissue culture and establishment of multiple shoot system etc were studied, and the key technologies ofcallus establishment and optimization in the process of establishing were discussed, which providedtechnical support for Arnebia euchroma (Royle) Johnst tissue culture breeding propagation. The resultsshowed as follows:1、The study was to establish the callus induction and its optimization of hairy roots from Arnebiaeuchroma (Royle) Johnst. The optimal combination of cytokinin and auxin was2,4-D0.1mg/L+IAA0.5mg/L+KT0.5mg/L; the optimal carbon source was30g/L glucose; the explant, most likely to induce thecallus induction, was the young leaves; the best combination of cytokinin and auxin for Callusproliferation was NAA1mg/L+6-BA0.5mg/L.2、The callus redifferentiation and its optimization of Arnebia euchroma (Royle) Johnst arestudied, the cultivation technique system as follows: the optimal combination of cytokinin and auxin wasIAA0.2mg/L+KT0.5mg/L; the optimal concentration of AgNO4was1mg/L; the optimal carbon source was30g/L sucrose; Compared with the callus from hairy root, the callus from leaf explants were more easilyforming callus rediferentiation.Adventitious bud proliferation and its optimization of Arnebia euchroma(Royle) Johnst were studied, the cultivation technology system as follows: The optimal combination ofcytokinin and auxin was TDZ1.5mg/L+NAA1mg/L; the concentration of AgNO4was0.75mg/L.3、Plantlets vitrification control in the tissue culture of Arnebia euchroma (Royle) Johnst werestudied, the cultivation technology system as follows: the ammonium nitrate concentration of NH4NO3was825mg/L; appropriate to reduce the concentration of IAA will effectively prevent the occurrence ofvitrification phenomenon; Hyperhydricity inverse conversion technology in the tissue of Arnebiaeuchroma (Royle) Johnst was studied, the cultivation technology system as follows:NH4NO3825mg/L.sucrose40g/L and agar10g/L.