Expressional Pattern Of SHP2on Postal Testicular Development At Different Ages And The Relationship Between Its Expression And Testosterone Level In Blood Of Mice

Testicular development is orchestrated by the integration of signaling inputs from proteins, hormones and growth factors. As one signal molecule member of the big family of protein tyrosine phosphatases, Shp2(Src Homology2Containing Protein Tyrosine Phosphatase2) involves various of cellular processes, including cell growth, cell differentiation, mitosis and oncogenic transformation. Moreover, in clinical research, Shp2mutation in genital cells is able to induce the abnormal development of testicles, including enorchismus, less amount of sperms and so on. Further research shows that Shp2is a key regulator of the blood testis barrier (BTB) integrity and Sertoli cell support of spermatogenesis and fertility. According to it, in this study, we analyzed temporal regulation rules of Shp2in mice testicles on different developmental stages on the transcriptional level by RT-PCR (both quality and quantity) and translational level by Western Blot. And then we identified the testosterone level in mice blood. Collectively our results showed the following conclusions:1. The temporal expression of SHP2gene in mice testis presented a successively regardless of the daily growth according to the semi-quantitative assay. Based on the results of qRT-PCR, it could be concluded that during the period of the twentieth day to thirty-first day, the expression quantity of Shp2gene fluctuated from the significant increase (P<0.01) to decrease (P<0.01) to increase again (P<0.01). And finally, the amount was back to normal gradually after the thirty-first day (P>0.05). Meanwhile, the expressional pattern of Shp2protein was basal in line with the pattern of its mRNA, which meant the protein of Shp2appeared during the whole developmental process. What deserved noticing were the significantly stronger expressional value on20th and31st days (P<0.05).2. By identifying the testosterone value in mice blood at different ages, the results showed that the testosterone level was relatively higher (670.67±21.866pg/ml) at birth. And then, the values declined and until20th, day became234.67±32.064pg/ml. On31st day, the sharp growth of the testosterone amount was onset and the first climax occurred 5277.64±51.460pg/ml of the46th day. However, this increase did not last longer. When the mice became body mature, the level went down to1676.56±23.398pg/ml. It namely indicated that between the birthday and20th days as well as between46th and60th days, testosterone fell while Shp2exhibited relatively stable expressions. Moreover, during the days of20to30, stronger expressional pattern happened with rocketing rise of testosterone. Due to this phenomenon, it is supposed that to define the effect of Shp2on testosterone value needs further investments.Collectively our results indicate that Shp2involves in the development of mouse testis, meanwhile the expressional pattern of Shp2is identical with the changing trends of testosterone during postnatal development of mice. But the functions in details need to be explored and investigated in depth.