| This paper mainly focused on the purification of recombinant proteins and theirbio-functions. The successfully purified of three recombinant proteins, Chz1, T2HBtzand Ras mutant RasG60A, together with the spectra study of Chz1-T2HBtzrecognition and NSC290956-RasG60A interaction had been described. The newlydiscovered intrinsically disordered histone chaperone protein Chz1and its binding toH2A.Z-H2B was investigated by using Circular Dichroism and fluorescencespectroscopy. It has been confirmed that Chz1undergo large conformation changedue to binding to H2A.Z-H2B dimmers. For the purpose of exploring its bindingdynamic process, we constructed the mutant T2HBtz Y139W, and explored itsfluorescence emission mechanism. The binding energy was also obtained usingsteay-state fluorescence spectroscopy to be30275.1±2558.6J/Mol. In addition, theinteraction of small molecule drug NSC290956and oncogenic protein mutantRasG60A has been investigated using Circular Dichroism, and the effectiveness ofcancer cell apoptosis has been verified in molecular level. |
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