Expression Profiling Of Rice Rnase T2Proteins In Leaves And Seedlings Under Salt-stressed Conditions

Background: Rice is one of the most important food crops in world, as well asone of the basic research model plants. Ribonucleases (RNase, RNS) T2are found inalmost all kinds of organisms, the conservation suggested the importance of theirfunctions. Of large-scale production of rice caused by salt damage, the impact of saltstress, involving almost all plant physiological and biochemical processes, leading toserious plant can not grow normally, cuts or even crops. Against salt stress mediatedsalt adversity molecular mechanism of rice production is of great significance. It hasbeen shown that the rice RNS family members play an important role in the processof growth and development and resilience.Objective: Preparation of the corresponding specific antibodies against rice RNSproteins that the genes encode, and then explore the expression mechanism of riceRNS gene coding proteins in leaves and seedlings under salt-stressed conditions,allthese provide clues and basis for research of function.Materials and Methods: Predict the epitope of the candidate genes codingproteins, and select the antigenic determinants peak higher fragment, synthesispeptides that coupled and immune New Zealand rabbits to prepare polyclonalantibody. Western blotting analysis was carried out for rice93-11material collected atdifferent developmental stages, further we examined their expression with salttreatment at different times points, and compared them to the leaf seedlingtranscriptional information in rice MPSS database for analysis.Results: We detected that the OsRNS1to OsRNS7proteins were expressed inleaves by western blotting, and OsRNS4protein was mainly expressed in seedlings,which indicated its function in seedling stage. The other proteins were expressedduring maturation stage, but we did not examine the expression of OsRNS8. Theexpression of OsRNS4was up-regulated under salt stress, whereas other RNSproteins were down-regulated with the extension of time. Comparison between theprotein expression abundance and transcriptional intensity revealed possiblecorrelations for some genes.Conclusion: Our research uncovered the expression profile of rice OsRNSsduring developmental stages and salt treatment. We found that OsRNS4expressed specifically during seedling period, which indicated that it may play a role in seedlingperiod, and we did not detect its expression after mature period. The other proteinswere mainly expressed in mature leaves, hardly in seedlings. Our experiment alsouncovered the expression pattern of RNS proteins in rice93-11with salt treatment.We discovered that the OsRNS4protein was up-regulated with salt-stress treatmenttime prolonged, indicating its characteristic function in salt-stress response process.OsRNS1protein was up-regulated four hours after treatment, but then down-regulated.OsRNS2、OsRNS5、OsRNS6、OsRNS7proteins were up-regulated within the first fewdays after salt treatment, soon down-regulated. This experiment provides valuableclues for understanding the functions of the RNS proteins in rice growth andsalt-stress process.