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Phase Behavior Of Mixed Binary Monolayers Consisted Of Amphotericin B And Sphingomyelin At Interface

Posted on:2014-01-02Degree:MasterType:Thesis
Country:ChinaCandidate:J J QiaoFull Text:PDF
GTID:2250330425954129Subject:Biomedical engineering
Abstract/Summary:PDF Full Text Request
With the development of modern science and technology, interdisciplinary theory and development technology integration and perfection, biology, physics, chemistry, Pharmacology and clinical contacts gradually highlighted and becomes the current topics in the field of science. Especially biofilm system has become a multi-disciplinary multi-field combination of typical and popular topics. Numerous studies indicate that complex biochemical reactions and the resulting life activities are inextricably linked with biofilm system biofilm. And simulation in vitro has become an important technical means to study the physical and chemical characteristics of the biofilm system, especially the mature Langmuir-Blogett (LB) film technology plays an important role in the study of the interaction between the drug and the biofilm. This article selected an important lipid molecules mammals-sphingomyelin (Sphingomyelin, SM) and amphotericin B (Amphotericin B, AmB),which is widely used in the treatment of deep fungal infections as the object of study.Cholesterol and sphingomyelin are two types of lipid molecules which is widely present in mammalian plasma membrane and important to maintain cell membrane structure backbone. Existing literature and research have explored the interaction between the different proportions or different structure of steroids and sphingomyelin that affects the parameters of lipid membrane such as structure flexible and soluble. Especially, metabolites of sphingomyelin involves in cell growth, differentiation, withered death and other important physiological activities. The role of sphingomyelin, sphingomyelinase and ceramide are closely related. Amphotericin B is a chiral polyene fungal antibiotics, which is an important drug for treatment of invasive infections, it primarily affects membrane permeability to inhibit growth of fungal. With significant renal toxicity of amphotericin B, it causes nausea, vomiting, loss of appetite, proteinuria and other side effects, so it is mainly used for the treatment of deep fungal infections of the lungs and other critically ill patients. And the clinical fungal infections obvious symptoms, skin or serum tests positive patients should not be optional. The reason of these symptoms and problems is not clear.This article through LB technique study amphotericin B and sphingomyelin phase behavior in the air-pure water interface and air-KCl solution interface, recording amphotericin B and sphingomyelin mixed molecular layer surface pressure and average molecular area curve (π-A curve). And we analyze the important parameters such as mean molecular area, excessive molecular area, modulus of elasticity, and thermodynamic characteristic on the basis of the π-A curve. Study the degree of integration between amphotericin B and sphingomyelin at the air-pure water interface and the air-KCl solution interface at the molecular level. Observe the surface morphology of the monolayer by atomic force microscopy (AFM), the results show that the surface morphology of the mixed monolayer is consistent with the results of analysis of the π-A curve.This paper research different molar ratio of sphingomyelin/amphotericin B mixed single molecular layer and use atomic force microscope observe the surface morphology of sphingomyelin/amphotericin B mixed monolayer at the air-pure water interface and air-KCl solution interface The results show that the interaction of amphotericin B and sphingomyelin has occurred on both interfaces of the interaction. Amphotericin B undermines the stability of the lipid monolayers, the basic trends are consistent, which proves the role of amphotericin B killing the fungal cells is closely related to the combination of amphotericin B and fungal cell membrane, and this may change the stability of the molecular structure and physicochemical properties of fungal membrane skeleton, so as to achieve a therapeutic effect.
Keywords/Search Tags:Amphotericin B, Sphingomyelin, LB films, AFM
PDF Full Text Request
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