Screening And Fermentation Of Microbial Strains Producing Lycopene

Lycopene has many physiological activities, especially in antioxidant. The microbialstrain producing lycopene was screened so as to realize the production of lycopene byfermentation, which was identified primarily to be Rhodotorula sp.In order to raise yield of lycopene of Rhodotorula, in this paper, the effects offermentation conditions on lycopene yields were studied. At first, select carbon, nitrogen,MgSO₄, Fermentation time, quantity of strain, pH, rotate speed, Fermentation Temperature,quantity of zymotic fluid, illumination as independent variable,biomass, content of lycopene,yields of lycopene as dependent variable, ake the single factor test.Secondly, select the timeand the quantity of blocking agent addition、the time and the quantity of H₂O₂addition asindependent variable,yields of lycopene as dependent variable, make another single factor test.As well as the orthogonal experiments of four factors three levels glucose, the yeast extract,MgSO₄, fermentation time orthogonal experiments, we have obtained the Rhodotorula’s bestfermentation condition glucose50g/L, yeast extract40g/L, MgSO₄0.03g/L, fermentation time108h; Fermentation temperature29℃, quantity of strain15%, pH6, quantity of zymoticfluid60ml, rotate speed160r·min-1; the yield of lycopene raise2times under theillumination;the time of blocking agent addition is60h, the quantity of blocking agentaddition is10mL/L, the time of H₂O₂addition is48h, the quantity of H₂O₂addition is1mL/L.Under this optimized condition,the highest yields of lycopene is8.56mg/L, raise67.29%.In the optimized of extraction process of lycopene,in order to raise the extraction rate, themethods of cell disruption of Rhodothece, extraction solvent, ratio of liquor and material,extraction temperature,extraction time and the number of extraction stage were studied, wehave obtained the lycopene’s best extraction condition:extraction solvent acetone: lightpetroleum=2:1, ratio of liquor and material1:10, extraction time30min, extraction temperature30℃, extract2times, in this extraction conditions,the highest content of lycopene is131.4μg·g^-1, raise30.9%.