Preliminary Investigation Of Diversity And Functional Enzymes Of Fungi From Erdos A Paleo Salt Lake In Inner Mongolia

The diversity of fungi in soils and sediments of Erdos paleo salt lake in Inner Mongoliaand the strains containing functional enzymes were identified. Three isolation mediacontaining designed concentrations of NaCl were used to separate and cultivate fungi. Basedon analysis of morphology and ITS sequences, the phylogenetic of tested bacterial strains wasperformed.2121strains of fungi were separated from the salt lake. These strains weredivided into45morphospecies and27generas based on the analysis of morphology and ITSsequences. The dominant genus was Aspergillus.The enzyme activity in the salt lake fungi was qualitatively determined using6kinds ofscreened media.Thirteen of45morphospecies produced protease(s),11produced cellulase(s),5produced compound enzymes,4produced amylase,1produced chitosan enzymes and1produced lipase. Meanwhile,4morphospecies could simultaneously produce three enzymesand3produced two enzymes. A strain of high-yield protease(s), name28b, and theconditions of the protease(s) production were optimized by single factor experiment andorthogonal experiment. Results show that the optimal conditions for producing protease(s)are30g/L peptone as nitrogen source,40g/L glucose as carbon source, initial pH7.0,inoculum size8%, fermentation temperature28°C and fermentation time60h. Under theseconditions, the enzyme activity is128.9U/mL. The fungi from the Erdos salt lake in InnerMongolia are rich in diversity and can produce various enzymes. This study provides a basisfor the further characterization and utilization of halophilic microbes.