Genomic Bioinformatic Analysis Of Draconibacterium Orientale FH5~T And Gilvimarinus Chinensis QM42~T

Draconibacterium orientale FH5T was isolated from marine sediment sample from coast of Weihai, China(latitude37°32’02.43" N; Longitude122°34’3.58"). Draconibacterium orientale strain FH5T (=DSM25947T=CICC10585T) is the type strain of Draconibacterium orientale gen. nov., sp. nov., a new species within the proposed new family Draconibacteriaceae in class Bacteroidiaby16S rRNA gene sequence similarity comparison and classification of multiphase studies. Due to Draconibacterium orientale strain FH5T is the type strain of Draconibacterium orientale gen. nov., sp. nov., Here we present a summary classification and a set of features for Draconibacterium orientale FH5T together with the description of the complete genome sequencing and annotation. We introduce a method for complete genome sequencing, which is building a database, sequencing, splicing, and then annotate and predict important genes and proteins, to analyze its function and possible mechanisms to achieve the purpose of digging its biological significance.This novel agar-digesting marine bacterium, whose genome is described here, was isolated from coastal seawater from an aquaculture site near Qingdao, China. Gilvimarinus chinensis strain QM42T is the type strain of Gilvimarinus chinensis gen. nov., sp. nov., a new species within the Gilvimarinus chinensis new genus. Gilvimarinus chinensis strain QM42T can degrade agar, which may play an important role in the bio-energy research. Here we present a summary classification and a set of features for Gilvimarinus chinensis strain QM42T together with the description of the complete genome sequencing and annotation.Complete genome sequencing of microbial genomes is now very mature. Microbial genome sequencing technology is very rapidly developing. Microbial genome sequencing has been widely applied to medical agriculture, biotechnology applications, biological evolution and ecology.Draconibacterium orientale FH5T and Gilvimarinus chinensis QM42T were high throughput sequencing. Experiment contains the following steps:DNA extraction, library construction, sequencing, splicing, gene annotation, etc. We use DNA Mini kit (TaKaRa Bio) to extract DNA and build a454Illumina library and a3K Solexa library. Gene sequencing is completed by454pyrosequencing and the Solexa sequencing-by-synthesis. Gaps were closed by sequencing PCR products, and low-quality regions were resequenced by primer walking. Annotating predicted genes is by blast, such as the NCBI, nr, COG and KEGG, etc.The total length of the data Draconibacterium orientale FH5T is83348428bp reads and the number FH5T is222320. The completed genome sequence of Draconibacterium orientale FH5T contains5132075bp, achieving an average of16-fold sequence coverage per base with error rate less than1in100000. The initial Newbler assembly consisting of152contigs in13scaffolds was converted into a phrap assembly by making fake reads from the consensus, to collect the read pairs in the454paired end library. G+C content of41.31%.Draconibacterium orientale FH5Tcontains4569open reading frames (Open Reading Frame, ORF), of which2214ORF (48.46%) with known functional protein sequence homology, and there are a lot of unknown function sequence marks the presence of a new gene and new functions. The predicted protein found Draconibacterium orientale FH5T strain genome can coding a variety of required enzymes and proteins such as the glycolysis, Krebs cycle, pentose phosphate pathway, oxidative phosphorylation, etc., which reflect the integrity of the metabolic pathways Draconibacterium orientale FH5T.The genome of Gilvimarinus chinensis QM42T consists of one circular chromosome with a length of4068866bp and G+C content of5955bp. Gilvimarinus chinensis QM42Tgenome sequencing steps be as same as Draconibacterium orientale FH5T. Gilvimarinus chinensis QM42T contains a plasmid with a length of Xbp. Gilvimarinus chinensis QM42T contains3824open reading frames (Open Reading Frame, ORF), of which2232ORF (48.46%) with known functional protein sequence homology, and there are a lot of sequences of unknown function. The predicted protein found Gilvimarinus chinensis QM42T genome can coding a variety of required enzymes and proteins such as the glycolysis, Krebs cycle, pentose phosphate pathway, oxidative phosphorylation, etc., which reflect the integrity of the metabolic pathways Gilvimarinus chinensis QM42T. Meanwhile, the genome of the strain contains8predicted agarase genes and three predicted alginate gene, during which is in accordance with the the result of the expriemen in the culture. The genes encoding these enzymes are relatively low sequence similarity with the known sequence of agarase and alginate, which reflects a strain of a novel agarase.