Study On The Agrocybe Aegerita Polysaccharide As Cigarette Humectant

Cigarette humectant is one of the important additives in cigarette industry, and plays an important role in moisture retention and improving sensory comfort of smoke. Cigarette humectant is also the part of vital technology for the characteristic of Chinese cigarettes. Fungal polysaccharides are a kind of active polymers which formed mainly by aldehyde group and ketone group through glycosidic bond, and show the the function as humectants. However, few researches have been focused on the fungal polysaccharides as cigarette humectants. The projects of this study were to i) screen a strain with high yield of fungal polysaccharide from edible and medicinal fungi, ⅱ) optimize the culture conditions to improve polysaccharide yield of the target strain, ⅲ) evaluate the application results of fungal polysaccharide as cigarette humectant, ⅰⅲ)clone two genes which coding the key enzymes involving in polysaccharide biosynthetic pathway of the target strain. Main results obtained in the present study were concluded as the follow:1. Agrocybe aegerita YMF1.02035, a mushroom strins producing high yield of polysaccharide, was screened out from10species of edible and medicinal fungi. The yields of intracellular polysaccharide (IPS) and extracellular polysaccharide (EPS) of the strain were3.61g/L and0.16g/L, respectively.2. Based on the polysaccharide yield, the medium MFM was determined as the suitable medium for A. aegerita YMF1.02035to produce polysaccharides, and the culture conditions for this strina were optimized as28℃,150rpm with an inoculation size of480mg/L. Under these optimal conditions, the maximum of polysaccharide yield producing by A. aegirit YMF1.02035was reached to5.23g/L at day9.3. The polysaccharide extracted from mycelia of A. aegirit YMF1.02035, HM2-ps, was more effective than the traditional humectant of glycerin regarding their functions of moisture retention and improving sensory comfort of smoke.4. Two genes, respevtively coding glucose phosphate mutase (PH) and UDP-glucose pyrophosphorylase (UD) in the polysaccharide biosynthetic pathway, were cloned from A. aegirit YMF1.02035by method of chromosome walking. The gene coding PH was determined as4358bp.