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The Effects Of Simulated Flooding On Cytochrome C Oxidase And Amylase Activity Of The Riparian Plant Arundinella Anomala Steud. In Three Gorges Reservoir Region

Posted on:2008-01-28Degree:MasterType:Thesis
Country:ChinaCandidate:D LiuFull Text:PDF
GTID:2250360215465989Subject:Ecology
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The formation of widespread water-fluctuation-zone in Three Gorges Reservoir Region will affect the eco-environment of this region when Three Gorges Project is completed. Vegetation construction can stablish banks and control soil erosion. As an important plant species chosen for constructing the vegetation in this region, Arundinella anomala Steud. has a developed roots system and strong flooding resistance, and it is widespread in Three Gorges Reservoir Region. Energy supply in plants affects their endurance of flooding tremendously, and cytochrome c oxidase and amylase are very important enzymes in energy production of plants.In this paper, four flooding simulation experiments were conducted to analyze the activity of cytochrome c oxidase and amylase in Arundinella anomala, they were short-term flooding experiment, long-term continuous flooding experiment, long-term intermittent flooding experiment and the experiment of recovery after flooding. In short-term flooding experiment, long-term continuous flooding experiment and long-term intermittent flooding experiment, three flooding treatment were set, included no flooding (control), underground submergence, whole plant submergence (top of the plants 2m under water surface). High frequency flooding (after every 9 days flooding, the plant has been exposed in the air for 1 day), low frequency flooding (after every 19 days flooding, the plant has been exposed in the air for 1 day) and no flooding (control) were set in the experiment of recovery after flooding. when the plant materials were been flooding for 0 hours, 8 hours, 24 hours, 48 hours, 72 hours in short-term flooding experiment, and been flooding for 0 days, 20 days, 40 days, 60 days, 80 days, 100 days in long-term continuous flooding experiment and long-term intermittent flooding experiment, and when the plant materials recovered for 0 hours, 8 hours, 24 hours, 48 hours, 72 hours and 96 hours after 40 days flooding, the activity of cytochrome c oxidase and amylase were test. The results showed that:(1) In short-term flooding experiment, when the plant materials were been flooding for 8 hours, the activity of cytochrome c oxidase had a rapidly rise in underground submerged plants and whole submerged plants to use the deficient oxygen. Then, the activity of oxidase in whole submerged plants decreased gradually. But the activity of oxidase in underground submerged plants kept a high level, because the underground submerged plants may transport oxygen from shoot to root. The response of amylase to flooding was slowly. Amylase activity in underground submerged plants showed no difference with controls, and the activity of amylase in whole submerged plants was higher than controls only after 72 hours flooding. The higher activity of amylase benefits produce more energy to compensate for lower energy production of anaerobic respiration.(2) Almost in all the long-term continuous flooding experiment, the activity of cytochrome c oxidase in underground submerged plants had no difference with controls. The cause of this also was the underground submerged plants may transport oxygen from thoot to root. Because the extreme deficiency of oxygen, the activity of cytochrome c oxidase in whole submerged plants was lower than that in controls. The amylase activity in whole submerged plants showed that Arundinella anomala Steud. had different strategies to cope with flooding in different stages. After 20 days flooding, the amylase activity in whole submerged plants was higher than controls, so that they can produce more energy through anaerobic respiration. From 40 days to 100 days, the amylase activity decreased. This decrease benefits to reduce the consumption of starch, and also is helpful to avoid cytoplasmic acidosis and self-poisoning by ethanol.(3) When flooding was over, the activity of cytochrome c oxidase and amylase showed a rapidly rise both in underground submerged plants and whole submerged plants. So, Arundinella anomala Steud. can produce more energy and recover from flooding quickly.(4) In long-term intermittent flooding experiment, the activity of cytochrome c oxidase in high frequency flooding treatment plants was lower than it in low frequency flooding treatment plants. And, after the exercise of intermittent flooding, amylase activity of high frequency flooding treatment plants was lower than it in low frequency flooding treatment plants at 80th day and 100th day. Amylase activity of low frequency flooding treatment plants was lower than it in controls at 100th day. These results showed that high frequency flooding can make Arundinella anomala Steud. have low energy consumption, and benefits to endure long-term flooding.From all the results, different flooding treatments have different effects on the activity of cytochrome c oxidase and amylase in Arundinella anomala Steud., and the plants with same treatment have different activities of cytochrome c oxidase and amylase at different times. All the differences and changes of the activity of cytochrome c oxidase and amylase benefits Arundinella anomala Steud. to cope with flooding.
Keywords/Search Tags:Three Gorges Reservoir, flooding, Arundinella anomala Steud., cytochrome c oxidase, amylase
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