SBR Reactor Of Removal Of Nitrogen With Bioaugmentation Technique

Low temperature may cause lots of problems to treating sewage by bio-treatmenttechnology, for the low efficiency and the high difficulty to reach the criterion. In coldarea, reducing sludge loading rates or enhancing the concentration of sludge orincreasingthe aeration rate are common solutions to these problems. Usingbioaugmentation technique can improve water quality, reduce cost and make the systemmuch steadier, without changing process parameters. Study onbioaugmentationtechnique used in low-temperature water-treatment is significant to the way and theratio low temperature engineering bacteria placed in, so as to thewater quality ofeffluentand the analysis to the community structure of activated sludge.Engineering bacteriaarescreened from one of wastewater treatment plants inHaerbin, includingnitrosobacteria,nitrobacteria and two aerobic denitrifying bacterias:Den-03, Den-5, which belongs to Pseudomonas psychrophila, Pseudomonas sp.Genbank number is JQ782901å'ŒJQ782902.The SBR reactors with Bioaugmentation Technique have better nitrogen removalefficiency than The SBR reactors without Bioaugmentation Technique. The results showthat in domestic wastewater COD removal rate is85%, NH₄⁺-N removel rate is65%,nitrate nitrogen is20mg/L; in artificial wastewater COD removal rate is90%, NH₄⁺-Nremovel rate is70%, nitrate nitrogen is15mg/L. The sludge with BioaugmentationTechnique MLVSS/MLSS can reach80%, Using Bioaugmentation Technique canaugment the sludge activity and get better sludge settling。Microbial Community in the reactors with Bioaugmentation Technique is diversityand rich than The SBR reactors without Bioaugmentation Technique. Through pilot testson omestic wastewater and artificial wastewater simultaneously, the difference ofactivated sludge can be found by comparing the two reactors. DNA abstractedfromactivated sludgeis used to continue study of microbial community and find thedifference of activatedsludge between domestic wastewater and artificial wastewater.The results show that main Microbial Community in the reactors with BioaugmentationTechnique is Alishewanella sp.（EU287929）, Pseudomonas psychrophila （JQ782901）,uncultured Paracoccus sp.（GQ985497）, uncultured Synergistetes bacterium（JQ346774）, uncultured bacterium （FJ672648）, Synergistetes bacterium （JQ346774） isDen-03.