Degradation Of Lignin By White Rot Fungi Enzymes And Screening Of The Triphenylmethane Degrading Strains And The Optimization Of The Degradation Conditions

Build white rot fungi degrade lignin enzymes-manganese peroxidase (Mnp) andpaint enzyme (Lac), the effects of substrate concentration, enzyme activity of the role ofthe degradation effect, the results showed that:1. Substrate concentrationlignin, theMnp system, alkali lignin concentration of40mg/L, the removal rate was29.41%, andthe Lac system, alkali lignin concentration of100mg/L, the removalthe maximum rateof62.67%;2different enzyme activity under the conditions of the lignin lignin removalrate of enzyme activity increases with the Mnp the Mnp system, increase the activity of3.6U/mL, the removal rate of18.40%, significantly higher than other Mnp degradationsystem, Lac system, the lignin removal rate with the Lac activity increases andincreased enzyme activity was3.6U/mL removal rate38.11%significantly impact thelevel of enzyme activity in Lac alkali lignin removal. On the degradation product wassubjected to GC-MS measurement, the results show that the the alkali lignindegradation product of some organic acids like substances, lipid substances andaromatic substances, etc., showed that effective degradation Mnp and Lac alkali lignin.Based white rot fungi can degrade complex substances containing benzene ringstructure (lignin), to examine its role in the degradation of triphenylmethane dyescontaining benzene ring structure, in order to expand the scope of application of whiterot fungi after Strains and identification has been the two degradation effect of a goodnew strains:1. strains F3, the bacteria were identified using morphological methods andITS method for Aspergillus niger (aspergillus niger); strains H4, identified trogii(Trametes hirsuta).Comparing two strains individually degradation and the effect of the mixeddegradation of dye, F3and H4mixed culture degrading dyes better culture72h afterdegradation of the removal rate of99.41%. Culture conditions optimized for results:speed160rpm, and substrate concentration of5mg/L, a temperature of30°C, pH=5degradation crystal violate removal efficiency.