Study On Qualitative And Quantitative Identification Of Animal Derived Material By Microsaellite Makers Technology

Lots of food adulterated, and imitationed enents take place around our life, which damaged the interests of consumers and affected the market order. Because of the outbreak of mad cow disease, people began to rethink the feasibility if animial-derived ingredients added into the feed. The identification of ingredients of animial origin became increasingly impport. With the development of molecular biology techniques, microsatellite DNA has been in-depth understanding, and widely used in population genetic studies,because it follows Mendel’s law of inheritance, can be stably inherited, but on the use of microsatellite DNA identification of meat. Establishing a rapid and effective identification of animal derived materials by microsatellite to determine the species became very important. For example:It can help impove the effective of food identification; rapid control of animal-derived free circulation of goods; expanding food safety control, to meet the needs of market development testing.This test identify the common animal-derived ingredients by use of microsatellite markers. Collecting pork, beef, sheep meat, goat meat, duck, goosen, chicken and dog meat as research objects. Picking out microsatellite markers of duck, pig, bovine and sheep from Gnenbank, designing primers based on microsatellite marker sequence, then then PCR-gel electrophoresis, screening intraspecies general, species specific microsatellite markers for identification.The results are as follows:In the identifacation of pigs, cattle, sheep, goats, ducks, goose, chicken, dogs animal derived components it can be used the duck’s specific microsatellite markers of AJ272583, which the amplified target fragments are267bp; the bovine’s specific microsatellite markers of AB463, which the amplified target fragments are178bp; the sheep’s specific microsatellite markers of x89268, which the amplified target fragments are207bp; the pig’s specific microsatellite markers of EF046, which the amplified target fragments are212bp. Make use of duck micro satellite markers of AJ272583, bovine microsatellite markers of AB463, sheep microsatellite markers x89268, pig microsatellite markers of EF046for fluorescence quantitative PCR. We can get the meat source component content of the formulas,the duck is:C=10[(Ct-17.77)/3.3844]×100%the beef is:C=10[(Ct-16.895)/-3.1275]×100%the sheep is:C=10[(Ct-18.629)/-3.2525]×100%the pig is:C=10[(Ct-19.389)/3.1439]×100%This method can provide a rapid and accurate way for detection and analysis of the adulteration of duck, beef, mutton or pig powder,which can be used as a new evidence for market supervision and administration.