Kinetic Resolution Of α-Amino Acids Based On Amino Acid Oxidase

α-amino acids have played an important role in current life science research and biomedical development, α-Amino Acids (α-AAs) are the major components of protein, and also the most important Amino Acids in the organism. The Amino Acids which constitute the protein in human body are all α-Amino Acids. In the20Amino Acids existed in the nature, excepting for glycine, all of α-Amino Acids are chiral, and respectively, play a unique function linked with people’s life closely. However, the number of natural amino acids is limited, and the non-natural amino acids are mostly racemic. D-enantiomers even play a different physiological role with L-enantiomers.In this article, we wish to resolute the α-racemic chiral Amino Acids by the method of biological catalysis. The organism that is used in bio-catalysis is mainly microorganism, and the essence of bio-catalysis is the use of microbial enzymes inside the cell to promote process of bio-transformation. The enzyme-catalyzed reaction is high specificity for substrates. This characteristic can be used to make one of the racemic isomers participate in enzymatic reactions to get another substance, while the other isomer is not affected. Therefore, the nature of the isomer left is significantly different from the consumption of substance. After that, the use of general physical separation can resolute of the two enantiomers.This experiment is divided into three aspects:1. Determining the enantioselective of microorganism for α-Amino Acid by screening30microorganism:DL-Met as a substrate, through the variety of microbial strains for the screening, resolute the D-Met and L-Met, and filter out the highly selective and efficient strains.2. The yeast (Rhodotorula graminis, R.g.) as the reaction strain:R.g. can provide L-amino acid oxidase(LAAO), and then we do the research of its substrate specificity, reaction mechanism, etc.3. Aeromonas sp. and HP0203which is broken (hpDAAO) as the reaction strains:both of them can provide D-Amino Acid Oxidase, and then we also do the research of their substrate specificity,reaction mechanism, etc.