Study On The Crafts About Walnut Peptide Nutrient Solution Made From Walnut Meal

As a result of the determination， there is33.33%walnut protein in the virgin walnut meal.However， the walnut meal is usually used to feed in China， which results in the protein and othernutrients being wasted. And the bioactive peptides has anti-oxidation， anti-hypertensive activity， whichmakes it a natural， safe and efficient functional foods.In this paper， walnut meal is used to raw material， prepares walnut polypeptide nutrient solutionwith antioxidant capacity by the enzymatic splitting method. The main results are as follows:(1) It uses the papain to enzymolysis walnut meal， uses Box-Behnken central composite to analysisthe optimize the hydrolysis conditions， and makes Hydroxyl radicals and superoxide anion radicalscavenging rate as response values， to get the optimal hydrolysis conditions are pH6， temperature50°C， time3h， substrate concentration3%. Under this condition， the respective rates of walnutpolypeptide scavenging hydroxyl radicals and superoxide anion radicals are32.7%and24.6%; Hydrolysisdegree is12%;(2) Using ultrasound-assisted enzymolysis， it analyzes the optimal conditions by Box-Behnkencentral composite:75%of the total power of the ultrasonic， ultrasonic time25min， temperature35°C.Under this condition， the respective rates of walnut polypeptide scavenging hydroxyl radicals andsuperoxide anion radicals are46.4%and38.8%; Hydrolysis degree is12.8%;(3) Using desalination method of mixed bed of anion and cation to desalt walnut peptide solution todetermine the optimum parameters for the desalination walnut polypeptide are: pH4.5， the speed ofpassed through8times column/h， cation-anion ratio3:2; It determines clearance rates of， the walnutpolypeptide to hydroxyl radicals and Chao Yang anion are respectively72.2%and65.3%，65.5%and59.7%，57.4%and50.7%by ultrafiltration determine， whose molecular weight are less than3kDa，3kDa-5Kda，5Kda-10Kda. At the same time， it makes a comparaton between the walnut polypeptidenutrient solutionand the antioxidant capacity of Comparison of Vc， to get the scavenging rates of thenutrient solution A， B， C grades and the undiluted solution of hydroxyl radicals and superoxide anion to Vc are75.0%and72.6%，68.0%and66.4%，59.6%and56.4%，48.2%and43.2%， which illustrateswalnut polypeptide nutrient solution has certain antioxidant;(4) With the analysis of software， the Conclusion of Optimal conditions about Stability is Xanthangum0.03%， CMC-Na0.025%， sucrose fatty acid esters of0.03%and the homogenizing pressure of35MPa. With these conditions， the best stability is97.2%;(5)By the analysis of Box-Behnken，the result is as followed: liquid ratio10%，sucralose0.005%，citric acid0.1%， walnut flavors0.1%. With these conditions， the highest score is89;（6）At121℃and0.1Mpa，under the condition of sterilization for10min，bottle filling，made intofinished products.Measured the various indicators，the results in line with national standards.Walnut peptide nutrient solution got from the above-mentioned test， has the strong ability ofantioxidant. At the same time， it provides some theoretical basis for the Industrialized production ofWalnut polypeptide.