Design, Synthesis And Spectra Study Of Colorimetric Probes Based On Rhodamine And Coumarin Derivatives

Fluorescent probe is an important method of identification, which has been widely applied in the field of biological imaging, diagnosis, treatment, optical probe technique and luminous materials, and has become as the efficient means of modern biotechnology and life sciences.Heavy metal ions plays an important role in medicine, food, biological and environmental fields. Due to its high toxicity, durability, easy mobility and bioaccumulation, Hg2+has become as one of the most high-profile environmental pollutants in the world.In the Chapter2, a new Hg2+probe was synthesized based on rhodamine B with1,2-diaminoethane and2,6-Pyridinedi-carbonyl dichloride. All the structural identification of products have been confirmed by1H NMR, and the sensing ability have been tested by UV-Vis spectrum. The results reveals that the probe shows highly selective to Hg2+in aqueous solution.Thiols play a key role in maintaining biological system, and have important biological significance. Cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) play important role in many physiological and pathological processes. Therefore, design and synthesis of thiol fluorescent probe for clinical diagnosis is very important.In the Chapter3, based on the high nucleophilic ability of thiols, a new thiol fluorescent probe has been developed based on new Michael acceptor. Its structural identification was confirmed by1H NMR and MS. The Michael addition between3-1and Cys was observable by1H NMR spectroscopy. To increase the response speed, we synthesed3-3through the reaction of3-1with CH3I. Time-dependent UV-Vis spectra of3-3with Cys shows that the reaction could finished within40s. Both two probes show highly selectivity and sensitivity to thiol.