Blood Lipid-lowering Function And Technology Of Producing Diacylglycerol Of Camphor Seed Oil

Cinnamomum camphora (Lauraceae), commonly known as one of tree species of the greatest economic value widely distribute in the south of the Yangtze River. The bitumen content of camphor seeds can reach40%, up to90%of total fatty acids were capric acid C10:0and lauric acid C12:0, both of which belong to medium-chain fatty acids. Camphor seeds have convenient source and huge resources, but their utility utilization is rarely. Therefore, we need to carry out research of systematicness for camphor seeds and camphor seed oil, at the same time, technological optimization of lipase hydrolyze camphor seed oil(CSO) product diacylglycerol(DAG), in order to facilitate their exploitation.With CSO as the raw material, using frozen sectioning, Nile red fluorescent dye and laser scanning confocal microscopy technology to investigate oil bodies and oil content of camphor seeds. To examine food safety of CSO from subacute toxicity test of SD rats. Study on effect of lowering blood fat by the CSO. Technological optimization of lipase hydrolyzed CSO product diacylglycerol. The results are as follow:1. Most of cotyledon storage cell of camphor seeds are polygons, less are ellipse, diameter of oil bodies in cells are (0.68±0.11)μm and more evenly distributed, some are oval or irregular shape, less of them are sphere. It can be obtained40%crude oil from hot pressing method and satisfactory refining CSO by filtration, deacidification, bleaching.2. In subacute toxicological test,30male SD rats were randomly divided into three groups, CSO group was fed CSO and soybean oil group was fed soybean oil and control group was only fed basic forage, performance in rats without any discomfort or death after35d. The result showed that no significant existed between CSO group and control group by hematology (red blood cell, white blood cell, hemoglobin et al.).3. Study on blood lipid-Lowering function,30male SD rats were randomly divided into three groups, using the hypercholesterolemia SD rats that through continuity fed basic forage with cholesterin and colalin to prepare high fat forage, control group was only fed basic forage and CSO group was fed CSO and soybean oil group was fed soybean oil, comparative study on the effect of CSO for blood fats of SD rats concerned with biochemical indicator, fat content and cellular structures in liver and aortic structure and using the hypercholesterolemia SD rats to observe the effect of antilipidemic and preventing and curving fatty liver and atherosclerotic of CSO. The results showed that Camphor oil could reduce the content of TC and raise the serum TG levels. Liver and aorta histopathology showed that the liver produced pathological changes, the size of cells were different and many lipids stacked in cells in soybean oil group, however, the liver produced little pathological changes and more lipids around cells, CSO could inhibit accumulation of lipid in aortic tunica intimae in CSO group. Conclusion:CSO has very significant effect of antilipidemic and preventing and curving fatty liver on the hypercholesterolemia rats.4. With camphor seed oil as the raw material, the craft of camphor seed oil to produce diacylglycerol (DAG) under the lipase hydrolysis was optimized. With pretreatment of organic solvents acetone and tert-butanol, the best lipase was chose among three commercial immobilised lipases including Lipozyme RM IM, Lipozyme TL IM and Novozyme435. Using quantities of DAG and Triacylglycerol as the response values, and setting the water content (30%-50%of enzyme mass), enzyme content (8%-12%of oil mass) and reaction temperature(55-75℃) as the variables, the response surface methodology (RSM) was applied to optimize the process of producing DAG by hydrolysis of camphor seed oil. The Results showed that Lipozyme RM IM was the best lipase with11reuse times after it was pretreated. The RSM optimal reaction conditions of DAG were:40%of water content,10%of enzyme load,65℃of reaction temperature, and under this condition, the quantities of both DAG and TAG were50.38%and40.68%, respectively. This craft can provide theory and test values for the application of Commercialized production of the Medium Chain DAG from camphor seed oil.