The Inhibition Of Three Antioxidants On AGEs And ALEs

Advanced glycation end products (AGEs) and advanced lipid peroxidation end products(ALEs) play an important role in the occurrence and development of chronic diseases, such asdiabetes and aging. The aim of this study is to investigate the inhibitory effects of threenatural antioxidants (tea polyphenols, rosmanol and licorice) on the formation of AGEs andALEs. Firstly, the Reducing Sugar-Amino Acid model system and Fatty Acid-Amino Acidmodel system are used to analyse the formation of AGEs and ALEs, respectively. Then thisstudy evaluates the extent of the advanced glycation and lipid peroxide reactions in two modelsystems by using spectrophotometry method, and measures the content of AGEs and ALEs byusing fluorescence spectroscopy method.The main results of this study are as follows:1. All three antioxidants significantly inhibit the formation of AGEs in the ReducingSugar-Amino Acid model system. Moreover, the inhibition rate is rising gradually withincreasing the concentration of three antioxidants, and there is a dosage-concentrationdependent effect.2. Tea polyphenols exhibits the best inhibitory effect on AGEs among the threeantioxidants. When tea polyphenols concentration is20μg/mL, the inhibition rate reaches78.71%. Furthermore, rosmanol and licorice have promotion effect on the formation of AGEsat low concentration, and have strong inhibition effect on the formation of AGEs at highconcentrations. When rosmanol and licorice concentration are100μg/mL, the inhibition ratereaches88.30%and94.75%, respectively.3. This study adopts the fluorescence intensity index to measure the content of ALEsthrough establishing Fatty Acid (50mmol/L, calculated with linoleic acid content) and AminoAcid (1mmol/L) reaction model system and adding tea polyphenols, rosmanol and licorice atthe concentration of25μg/mL-200μg/mL to the reaction model system. The results show thatall three antioxidants have significant inhibitory effects on fluorescence ALEs at roomtemperature and37C. Moreover, when the concentration is200μg/mL and tea polyphenols,rosmanol and licorice extract are incubated for12days at37C, the inhibition rate is73.1%,38.3%and72.9%, respectively. At90C, the inhibitory effect of the three antioxidants on ALEs is weak.In a word, tea polyphenols, rosmanol and licorice can inhibit the formation of AGEs andALEs under the experimental condition.