Tibetan Medicinal Lvluohua Main Active Ingredient Extraction And Research

Lvluohua is tibetan medicinal,Alias is pothos、stone ganzi、horseshoe gold,Latin name is Araceae. Lvluohua produced in the cold region of Tibet Himalayas(country of central and south america), Flowering in july-august of each year, about30days flowering. Lvluohua have strong aroma and slightly cold, Lvluohua can behypoglycemic and hypolipidemic. Lvluohua has therapeutic effect in diseases such asdiabetes、hypertension、coronary heart disease、vascular inflammation.Based on the orthogonal experiment and extraction agent used by ethanol,themethod of ethanol refluxing extraction is employed to extract the flavonoids inLvluohua and spectrophotometry is manipulated to determine the flavonoidcontent.The study shows that the power of the three factors in influencing theflavonoid extraction from Lvluohua declines from liquid-solid ratio to extraction time,and to ethanol concentration. In the optimal extraction process, the liquid-solid ratio is1:12, extraction time is90min and ethanol concentration is75%. In this way, theflavonoid content in Lvluohua can reach0.75%.Extraction polysaccharides from Lvluohua by hot water extraction. Withreagent blank as the reference solution. Absorbance value was determined by uv-visspectrophotometer, total polysaccharide content are obtained,and design orthogonalexperiment,Know the conclusion by Poor analysis method of orthogonalexperiment.the power of the four factors in influencing the Polysaccharides extractionfrom Lvluohua declines from temperature to extraction time, to liquid-solid ratio. andto number of times. In the optimal extraction process, the temperature is95℃,extraction time is240min, the liquid-solid ratio is1:60and number of times is three.In this way, the Polysaccharides content in Lvluohua can reach9.363%.Microwave digestion Lvluohua and agent used by concentrated nitric acidand Hydrogen peroxide. Extraction trace metal elements from Lvluohua by method ofAAS. digestion Lvluohua use concentrated nitric acid,Constant Lvluohua’svolume used by concentrated nitric acid of1%.Extraction Trace metal elements fromLvluohua by method of ICP-MS. two methods Results show that the order in Lvluohua of each element Content is Mg>K>Ca>Na>Mn>Zn>Cu，in this twomethods， Not detected following elements:V、Cr、Fe、Go、Ni、Ag、Cd、Hg.Extraction essential oil from Lvluohua by Supercritical carbon dioxide fluidextraction. Identificate chemical composition used by method of GC-MSD.32peakwas isolated and identified32compounds from volatile oil of Lvluohua.Threecompounds were higher than others content,this compounds are N-hexadecane acid(41.039%)、linoleic acid (13.901%),and alpha linolenic acid (9.396%).Select aluminum crucible. Analysis Lvluohua and Sample of not Containflavonoids by TGA. Draw the map of thermal gravimetric analysis. Drawn twosample dehydration process、Volatilization of the volatile components、Organicdecomposition process、Glass transition、Carbonization process and sample mass lossdata. Through the melting peaks in the spectra to judge the whole process is anendothermic process. The sample need to absorb heat during decomposition tocomplete phase transformation and the bond breaking.