The Research Of Bagasse Mixed With The Molasses Yeast Wastewater By Solid Fermentation

Molasses yeast wastewater mainly comes from the process of yeast fermentation broth separation and molasses pretreatment, It is a high concentration organic wastewater, the color is a deep tan, which is difficult to be degraded by natural microbes, lightfastness and high temperature resistant, its color has no obvious change as the placed time prolong; meanwhile, The biochemical characteristics of this part of the wastewater is low Ph, high COD and high BOD, the biochemical treatment technology is difficult to deal with emissions discharge standards.However, the waste water is rich in nitrogen, phosphorus, potassium, calcium, iron and other inorganic trace elements and humic acid, fulvic acid and other organic matter, it Can be used for fertilizer, feed additives. Direct emissions is not only waste valuable resources, but also cause serious environmental pollution, the destruction of the ecological balance.concentration is one of the widely used technology in molasses yeast wastewater treatment, Concentrated material as fertilizer could easily lead to soil compaction and other issues. Increasing lack of resources today, if the valuable material can be recycled from concentrated liquor to produce higher-value products, which not noly can be reduced yeast wastewater follow-up processing load But also has significant social and economic benefits. The issue is in the point of the recycling of the waste water, and finding a new treatment processes of molasses yeast wastewater; Use bagasse to adsorb potassium recycling wastewater, using Bacteria mixed solid fermentation to Product roughage. It provides a theoretical basis to explore the new ways for managing molasses yeast wastewater. The main contents of research and the results are summarized as follows:Determination of the main components in molasses yeast wastewater. Determine Protein Content by kjeldahl determination, determine total ash by Sulphated ash method, determine the content of residual sugar by Phenol sulfuric acid method. As a result, the pH=4.8, Protein Content is88mg·mL-1, the total ash is285.24mg·mL-1. The total sugar is359.89mg·mL-1, amino nitrogen is7.01%,53.1°x, total solids is730.9mg·mL-1. This suggests that the content of crude protein, solids, and inorganic salts in molasses yeast wastewater are relatively high, having great development potential.We studied ZGC108exchange resins separated K+in molasses yeast wastewater, and used dilute sulphuric acid to elution K+:put the exchange capacity as target to optimize the sample rate, temperature, K+concentration of yeast liquid waste and packing height; Put K+elution rate as target to optimize height-diameter ratio, temperature and flow rate of eluent. Meanwhile, we analysed adsorption capacity of regenerating resin. The sulfuric acid solution concentration was0.6mol·L-1, elution speed was3BV·h-1, the temperature of the eluent was35℃, in this condition, elution rate could be achieved99.5%. Experiments shew that, adsorption rate of the three times regeneration of resin still had74.68%, could use repeatedly.We studied the technology of bagasse adsorbed molasses yeast wastewater so as to produce fermented feed. Through single microorganism experiment, we sift trichoderma viride (T-6), aspergillus niger (A-2), protein candida yeast (C-2) and brewer’s yeast (S-2) to match; Trichoderma viride (T-6) to aspergillus niger (A-2) to protein candida yeast (C-2) ratio is2to2to3, bagasse to soybean meal ratios7to3, solid-liquid ratio is1to4, the crude proteins can reach15.4%by bagasse adsorption molasses yeast wastewater solid-state fermentation. With the orthogonal optimization experiment, the optimal fermentation condition: temperature is303K, time is5d, inoculate amount is1%and nitrogen is5%. At this point the total proteins in the culture medium can reach18.6%. Pretreatment of dry material was examined:through30mesh sieving, the culture which obtained by dry materials adsorption molasses yeast wastewater and with high pressure sterilization for1h was conducive to the growth of phallus and the increase of proteins.