The Study Of Kinetic Evaluation Of Coupling Process For Nitrogen Removal From Swine Wastewater And Hydrogen Sulfide Removal From Biogas

Does the reaction rate of swine wastewater anaerobic digestive juices aerobic post-treatmentdenitrification with biogas desulfurization coupling process depend on the reaction rate of themicroorganism or the mass transfer rate of biogas hydrogen sulfide to the mixed liquor? One of thetasks to answer this question is to understand denitrification and desulfuration decoupling link microbialreaction rate and its influencing factors. Under the support of the natural science foundation ofwastewater denitrification and biogas desulfurization of coupling reaction dynamics (No.31101749),artificial simulated nitrogen sulfur-containing wastewater as the substrate, the substrate concentrationand temperature on the denitrification and desulfuration coupling microbial nitrogen conversion rate andthe influence of the reaction kinetics parameters. The main research results as followed:(1)Four different ways cultivating domesticated denitrification and desulfuration microbial process,NOx-N removal rate of inoculated anaerobic sludge reactor is more stable than that of inoculatedaerobic sludge reactor.The biogas reactor (21d) of H2S for substrates needed more time for sulfideconversion rate reached60%than the reactor (3d) of nitrogen sulfur-containing wastewater as thesubstrate.No inoculated sludge and nitrogen and sulphur wastewater filling reactor can alsodomesticated desulphurization and denitrification sludge, just need more time to tame.(2)Denitrification and desulfuration reactor interrupted operate again after2months to makesludge activity recovery, H2S in the biogas and nitrogenous wastewater as substrate culturedomestication sludge need15d to recover sulfur conversion activity,more than nitrogensulfur-containing wastewater as the substrate of domesticated sludge. The other three domesticatedways sludge performance is almost in the entire active recovery process.(3) Denitrification and desulfuration sludge microorganisms of dominant population is analysisedthrough DGGE of molecular biology, the research have achieved good separation effect, and showedfully the four kinds of domestication method coupling sludge denitrification and desulfuration system inmicrobial diversity were identified that Mr Bush’s-Shigella genera (Escherichia-Shigella).(4)Under the condition of15℃,20℃,25℃,30℃to35℃, the substrate concentration on thedenitrification and desulfuration coupling the effects of microbial transformation of nitrate nitrogen rate,the dynamics model was established.15℃,20℃,25℃,30℃and35℃of the nitrate nitrogen conversionrate is respectively4.08d-1,4.98d-1,5.66d-1,6.22d-1and7.16d-1;relative to nitrate nitrogen saturationconstant were3.06mg/L,3.43mg/L,3.79mg/L,4.37mg/L,4.55mg/L.Nitrate nitrogen transformation rateand subsaturation constant increased with a rise of temperature, the temperature effect on the rate ofnitrate nitrogen conversion coefficient is1.03;The temperature coefficient to half-and-half saturationconstant the rate of nitrate nitrogen conversion is1.02. (5) Under the condition of sludge concentration in reactor was3.6g/L, nitrate nitrogenconcentration was2.72mg/L, calculate the15,20,25,30and35℃the nitrate nitrogen conversion ratecould respectively reach is6.92,7.91,8.49,6.92and7.91gNO-3-N/(L·d).(6) Under the condition of15℃,20℃,25℃,30℃and35℃,the substrate concentration on thedenitrification and desulfuration coupling the effects of microbial transformation of denitrification rate,the dynamics model was established.15℃,20℃,25℃,30℃and35℃of the denitrification conversionrate is respectively16.9d-1,27.6d-1,39.5d-1,81.3d-1and127d-1; relative to denitrification saturationconstant were4.52mg/L,6.43mg/L,8.26mg/L,14.2mg/L,20.0mg/L.Sulfide transformation rate andsubsaturation constant increased with a rise of temperature, the temperature effect on the rate of sulfideconversion coefficient is1.11;The temperature coefficient to half-and-half saturation constant the rate ofsulfide conversion is1.08.(7)Under the condition of sludge concentration in reactor was3.60g/L,hydrogen sulfideconcentration was0.049mg/L, calculate the15℃,20℃,25℃and35℃temperature conversion rate couldreach sulfides were5.23,5.34,5.64,5.82and5.85g S2-/(L·d).