| In recent years, rare earth up-conversion（UC） luminescent labeling materialshave attracted much attention due to their unique physical and chemical properties.One of the hot research topics in UC nanomaterials is to find out some novel efficientsynthesis methods for improving the UC luminescence efficiency. Among the varioushost materials, NaYF4matrix is one of those that have the highest UC luminescenceefficiency due to its low phonon energy. Herein, NaYF4matrix material is chosen. Inorder to further improve the UC luminescence efficiency, Gd3+is introduced.Solvothermal method is utilized to synthesize water-soluble NaY（Gd）F4: Yb3+,Er3+nanoparticles, which are used as luminescent probes to realize the sensitivedetection of proteins. The main research contents are as follows:Water-soluble NaY（Gd）F4: Yb3+, Er3+nanoparticles were obtained bysolvothermal method. The influence of Gd3+doping concentrations on the structure,morphology and luminescent properties of the UC nanoparticles were investigated.The results indicated that Gd3+favored the phase transformation from the cubic phaseto the hexagonal phase. And the particle size increased gradually at first anddecreased afterwards with the increase of Gd3+doping concentration. The UCluminescent efficiency was related to the crystallographic phase and the size ofnanoparticles. More hexagonal phase could enhance the UC luminescence, while thedecrease of the particle size would reduce the luminescense. The increasing of thereaction time also facilitated phase transformation, thus enhancing the UCluminescence. Through the adjustment of the reaction parameters, the pure hexagonalphase of NaY（Gd）F4: Yb3+, Er3+was finally obtained.Utilizing the Glutaraldehyde method, NaY（Gd）F4: Yb3+, Er3+UC nanomaterialswere coupled with monoclonal antibodies. The sandwich-structured biodetectionstrategy was designed. High sensitive detection of proteins was achieved using the UCluminescence as the detection signals. |
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