The Synthesis Of Carbon Nanoparticles (CNPs) And The Interaction Between CNPs And Biological Molecules

Carbon nanoparticles (CNPs) is a novel fluorescent nanomaterials. It has become a research focus because of its stable fluorescent property, small molecular weight and particle size, and non-toxicity in bio-system.The main contents of this paper are as follows:1. CNPs were synthesized from glucose via hydro-thermal method. They have small particle size, high fluorescence intensity and quantum yield, uniform distribution and high stability in water. In this method, synthesis and modification can be completed at the same time more simply, carbon quantum can be purified more easily, and the fluorescence quantum yield is3-5times of other synthetic methods.2. The interaction between CNPs and histidine (His) was studied. The results indicated that the fluorescence intensity obviously increased after CNPs combining with His. So did the fluorescence quantum yield and the photoluminescence stability. In order to obtain the highest fluorescence intensity the reaction temperature would be controlled at100℃, the environment of acid and basic should be at the isoelectric point of histidine, namely pH7.59, and1as molar ratio of His to CNPs.3、The interaction between CNPs and macromolecules such as Trypsin and BSA were investigated respectively. Both Trypsin and BSA could react with CNPs. The fluorescence intensity, particle size and photoluminescence stability increased or enhanced after their conjugating.