Mechanism And Difference For Pb And Cd Tolerance And Accumulation In Typha Angustifolia Of Two Ecotypes

The aquatic plant restoration of heavy metal pollution of the environment has becomean important research aspect in molecular biology and environmental science in recentyears. But due to the lack of difference in tolerance and accumulation of heavy metals byplants and its mechanism of action, the application of phytoremediation technology forheavy metal pollution is constrained. through the field survey, Typha angustifolia, theperennial aquatic plants, are found in Huaihe River and Chaohu Lake area, which have noheavy metal poisoning symptoms, large biomass and vigorous growth. This article selectsthe aquatic plants Typha as the test materials, using the pot experiment, comprehensivedealing with the wastewater irrigation method, morphological observation, enzymatichydrolysis, the overspeed centrifugal technology, analytical techniques of flame atomicabsorption spectrometry, chemical and solvent extraction, enzyme activity analysis andother methods to determine the corresponding physiological biochemical index, andpreliminary discussed differences for two different ecotype Typha in heavy metal toleranceand accumulation of lead, cadmium and its mechanism of action, which provide sometheoretical basis for further in-depth study.The experiments showed that under the condition of pot experiment, compared withcontrols, two different ecotype Typha plants’ body growth, physiological and biochemicalindexes have changed for lead, cadmium, sewage water treatment. During the test, Typhaplants were poisonous to some extent, and with the increase of wastewater concentration oflead, cadmium poisoning symptoms also be significantly enhanced. Chaohu Lake Typhaplants’poisoning symptoms were more serious than the Huaihe River Typha plants. Leafgrowth rate showed a trend of decrease after the first rise and the Huaihe River Typhatolerability of lead is11%higher than Chaohu Lake cattails,15%higher than that ofcadmium tolerance. The activity of SOD and CAT was increased with concentration of lead,cadmium wastewater to reduce instead. Absorption content of heavy metals in Typha plantsfor root> leaf were increased with the increase of the concentration of processing. Bytolerance analysis of sensitive lead concentration tendency for0.9mmol/L train testunder sewage irrigation, lead content in Huaihe plants’ root and leaf were all higher than inChaohu,28.4%,16.1%respectively. By tolerance analysis of sensitive Cd concentrationtendency for132μmol/L train test under sewage irrigation, lead content in Huaiheplants’ root and leaf were all higher than in Chaohu,22.9%,16.7%respectively. Heavy metal transport coefficients of two ecotypes have a trend of increasing, are less than1,which illustrates that the most of heavy metal accumulation was accumulated in roots, andreduced the toxicity of aboveground organs. To some extent, which improved Typhatolerability for lead, cadmium. In pot experiment with the increase of wastewaterconcentration of lead, cadmium, the content of GSH increased, which showed that heavymetal induced the expression of certain genes, may be a mechanism of plant against heavymetal stress. This also provided a theoretical basis for improving the heavy metal resistanceof plants through genetic engineering.