Study On Preparation And Application Of Dibutyl Phthalate Molecular Imprinted Polymer

Dibutyl phthalate is a kind of plasticizer which used to increase the elasticity ofPVC. It is belonged to the phthalic acid esters plasticizers.They are poisonous tohuman body such as carcinogenic,caused to deformities and genetic mutations etc.Due to between plastic substrate and phthalic acid esters was not formed chemicalcovalent bond, so it is easy to dissolve out from plastic products into the environmentor food. Now, phthalic acid esters ’s analysis method relies on the former enrichmenttechnology, such as liquid-liquid extraction, solid phase extraction, and then by GC orHPLC or GC-MS measure the concentration of phthalic acid esters. Because of thecomplexity of the tested phthalic acid esters in the sample matrix, which limits itsapplication.Molecular imprinting technology is a new technology which received a highdevelopment in recent years. It has wide applicability, specific identification,structure reservation in advanced, etc. Solid-phase extraction can together toenrichment and separation, It has the characteristic of simple operation and low cost,high efficiency, higher adsorbability and lower organic solvent consumption etc, butthe specificity is poor. Molecularly imprinted polymer as SPE separation medium canmake up for the shortage, it can separate mixtures of structure similar, can also beused for enrichment and purification of a target in the complex system, so as to realizeaccurate, rapid and efficient of the target compounds content determination.1.Using molecular imprinting technology, with dibutyl phthalate(DBP) as thetemplate,through bulk polymerization prepared the molecularly imprinted polymer ofDBP. The structure of molecularly imprinted polymer were characterized.Optimizingits preparation process conditions and the technological conditions as follows: MAA asfunctional monomer, EGDMA as the crosslinking agent, chloroform as solvent, andthe template moleculers(DBP): functional monomer(MAA): crosslinker (EGDMA)=1:3:30. On this basis, through the static adsorption experiment studied dibutylphthalate molecularly imprinted polymer’s adsorption equilibriumperformance,dynamic and selective adsorpion to dibutyl phthalate. The absorptiondynamics indicate that Lagergren level2equation can better characterize themolecularly imprinted polymer to DBP’s adsorption. The isothermal adsorptionindicate Langmuir isotherm equation can better describe the molecularly imprinted polymer to DBP’s adsorption. Selective adsorption showed that molecularlyimprinted polymers has good performance to DBP,The recognition factor is1.792. With dibutyl phthalate as template, through precipitation polymerizationmethod, the dibutyl phthalate molecularly imprinted polymer microspheres wereprepared.and then characterized the molecularly imprinted polymer microspheres. wealso studied the molecularly imprinted polymer microspheres’s selectivity andadsorption performance to dibutyl phthalate. The absorption dynamics indicate thatLagergren level2equation can better characterize the Molecularly imprinted polymermicrospheres to DBP’s adsorption. The isothermal adsorption indicate Langmuirisotherm equation can better describe the Molecularly imprinted polymermicrospheres to DBP’s adsorption.3. Used the molecularly imprinted polymer(MIP) of dibutyl phthalate as astationary phase medium prepared molecularly imprinted SPE column, Optimizationof solid-phase extraction’s washing and elution conditions,and then directly used toextract the dibutyl phthalate from milky tea’s extractant. after loaded sample,usedmethanol-water(volume ratio1:9) mixture solution to washing, and then usedmethano-acetic acid(volume ratio9:1) to eluted, the results found that through extractcan effectively separate and enrich of dibutyl phthalate from milky tea’s extractant.