Study Of The Interaction Between Three Kinds Of Anticancer Drugs And DNA And Cancer Cells Based On Iron Nanoparticle Probe

Cancer is one of the world’s highest death rates disease, people have been searching fornew effective method and medicine to treat cancer for a long time. In recent years, studieson the interaction of drugs and the tumor cells at the molecular level has become a newhotspot in researches of the mechanism of anticancer drugs. Many new anticancer drugstargeted cancer cells DNA, through a variety of ways to combine with DNA, can influencethe process of DNA replication and transcription, and affecting the proliferation and growthof cancer cells.In this paper, we used citric acid water-based Fe₃O₄magnetic nanoparticles as aspectral probe, to construct the nano-probe/anticancer drug/DNA three element systems.Combining with the resonance light scattering technique, UV-vis absorption spectrum,fluorescence spectroscopy, viscosity and other chemical analytical methods, we studed theantitumor mechanism of three classes of anticancer drugs targeted to DNA on the molecularlevel in vitro. And nano-probe and anticancer drugs on MCF-7breast cancer cells wereobtained by MTT method to get IC50, the nanometer probe and combined with anticancerdrugs on apoptosis of Hela cervical carcinoma cells, MCF-7human breast cancer cells,MHCC97-H cells, further studed on inhibitory effect of drugs on cancer cells on cell level.Study the interaction between four kinds of anthraquinone anticancer drugs:Mitoxantrone, Epirubicin, Daunorubicin, Adriamycin and DNA, respectively, and theinteraction between nano-probe/anticancer drug/DNA three element systems were discussedin detail. Citric acid water-based Fe₃O₄magnetic nanoparticles and anthraquinoneanticancer drugs is formed by electrostatic interaction and hydrophobic interaction of theaggregates, embedded in DNA and formed supramolecular compounds.Through various kinds of chemical analysis methods and cells experiments, theinteractions of three kinds of the natural antibiotic anticancer drugs: Bleomycin, BleomycinA5, Mitomycin C and DNA have been studied, and the nano-probe/anticancer drug/DNAthree element systems have been built. In the presence of Fe₃O₄,Bleomycin and BleomycinA5induced DNA scission by oxidative DNA; Mitomycin C combined Fe₃O₄became asalkylating agent, then cross-linking with DNA. The three kinds of drugs all could induceapoptosis of Hela cervical cancer cells, MCF-7breast cancer cells and MHCC97-H livercancer cells. Cells experiment results were similar to the experimental results of chemicalanalysis. Studying on three alkylating anticancer drugs: Aimustine, Semustine, nitrogen mustardinteracted with DNA. Combining with a variety of spectroscopic experiments and cellbiology experiments, nano-probe/anticancer drugs/DNA system were discussed in detail.The effect of the three kinds of drugs on DNA will cause the DNA spectra and viscositychanges, cell experiments showed that these drugs can promote Hela cervical carcinomacells, MCF-7breast cancer cells, MHCC97-H liver cell apoptosis, the cells experimentalresults and the spectra experimental results are about the same.