| Vaeonia ostii t. Hong etJ. X. Zhang, as a category of Paeonia suffruticosa in Tongling, has important nutritional and pharmaceutical value. The root of Paeonia ostii t. Hong et J. X. Zhang is a kind of famous traditional Chinese medicinal materials in Anhui province. The resources of Paeonia ostii t. Hong et J. X. Zhang seeds are abundant, and up to now most of the Paeonia ostii t. Hong etJ.X. Zhang seeds have not been exploited. In the paper, It was studied that antioxidant ingredient of Paeonia ostii t. Hong et J. X. Zhang seeds and its extraction technology. Then, the extraction, antioxidant activities and antibacterial activity of flavonoids which are active ingredients from Paeonia ostii t. Hong et J. X. Zhang seeds as raw material were studied. The main results were as follows:1. The results showed that the average content of fats was34.06%, starch20.25%, reducing sugar12.28%, protein12.03%, water11.46%and ash2.64%.2. The antioxidant activities of the alcohol extract were evaluated in total antioxidant activity of ascorbic acid system and2,2-diphenyl-l-picrylhydrazyl (DPPH) system. The antioxidant activity of alcohol extraction from Paeonia ostii t. Hong et J. X. Zhang seeds, soybeans, peanuts, sesames and pine nuts were compared in the above two systems, the results showed that Paeonia ostii t. Hong et J. X. Zhang seeds has the more potent antioxidant activity than other four plants. The optimum conditions of alcohol extract antioxidant ingredient were alcohol concentration70%, solid-solvent ratio1:20, extraction time3h, temperature25℃in total antioxidant activity of ascorbic acid system. The optimum conditions of alcohol extract antioxidant ingredient were alcohol concentration70%, solid-solvent ratio1:20, extraction time4h, temperature25℃in DPPH system.3. The result showed Paeonia ostii t. Hong et J. X. Zhang seeds ethyl acetate extract had the strongest antioxidant activity and the most contribution to the antioxidant activity of alcohol extract, and the contribution rate was71.96%. The total flavonoids content of the ethyl acetate extract was6.206mg/g, and the rate of alcohol extract was69.46%, which was approximate at the antioxidant contribution rate. So total flavonoids might be one species of antioxidant ingredient in Paeonia ostii t. Hong etJ.X. Zhang seeds.4. This paper focused on total flavonoids extracting from the Paeonia ostii T. Hong et J. X. Zhang seed to optimize the extracting procedure. By the Box-Behnken response surface analytical method, with the extraction rate of total flavonoid as index, the ultrasonic-assisted extraction of total flavonoids from the seed was investigated. The optimum conditions of flavonoid extraction were as follows:alcohol concentration75%, solid-solvent ratio1:21g/mL, ultrasonic extraction time49min and ultrasonic power240w, the extraction rate of total flavonoids was92.53%.5. Studied on the antioxidation of Paeonia ostii T. Hong et J. X. Zhang seed flavonoids in the paper. It showed that total antioxidant activity linearly responded to the flavonoids concentration. IC50of DPPH scavenging rate was24.35mg/L. The superoxide anions ability was up to47%. Using antimicrobial assays against four microbes, the results indicated that the Paeonia ostii T. Hong et J. X. Zhang seed flavonoids had antimicrobial activity against Bacillus subtilis and Salmonella during the range of the experiment, the inhibitory concentrations for the growth of Salmonella were5%-20%, and the inhibitory concentrations for the growth of Bacillus subtilis were20%. However the Paeonia ostii T. Hong et J. X. Zhang seed flavonoids have not antimicrobial activity against Escherichia coli and Aspergillus nige during the range of the experiment. |
PDF Full Text Request