Scanning Of Protectants Against Oxidative Damage And Bone-loss Induced By Simulated Space Environment And Research On The Protective Effects Of G-B-R

With the continuous development of manned space technology, ionizingradiation and microgravity effects of space have become an important factor.Natural products have abundant resources and distribute widely. Natural productsnot only contain the necessary proteins, lipids, carbohydrates, but also possess avariety of vitamins, minerals, active enzymes, growth hormones and so on, whichwere called “comprehensive micro-nutrition stores” and played important role inhuman growth and development. A variety of natural products were extracted bysophisticated extraction technology in this topic, and the in vitro antioxidant, bonemarrow mesenchymal stem cell proliferation, alkaline phosphatase activity ofosteoblasts and intracellular free calcium concentration index were selected toscreen stronger anti-radiation and anti-bone loss products.The selected productswere mixed according to the requirement dosages of national health care product,and then investigated by the animal experiments. Finally, the compositions of theproducts were analyzed. The present study provided advice and ideas fordevelopments of extreme special environmental protestant, aerospace food,radiation protection of special trades people and daily care for human.The maincontent as follows:The screening of anti-radiation materials: in vitro antioxidant activities andbone marrow mesenchymal stem cell proliferation of water extracts and60%ethanol extracts from14kinds of materials were compared. Results showed thatbuckwheat pollen, ginkgo biloba, blueberries, angelica and astragalus possess thestronger activities respectively.The synergies comparison of anti-radiation natural products: In vitroantioxidant (OH·, DPPH and total reduction capacity) and promoting bone marrowstem cells proliferation activities of10kinds of mixed products were determined.According to the CI index, Ginkgo biloba-pollen complexation has the strongestanti-radiation effect.The selection of anti-bone loss material: The osteogenic cell proliferationactivities, alkaline phosphatase activities and the effect of intracellular free calciumconcentration of12kinds of material extracts were investigated. The resultsindicated that the extract of Rehmannia has the best anti-bone loss activities.Through the establishment of bone loss model by cell gyroscopes, the researchfound that the extract of Rehmmannia could effectively promote the bonemetabolism related enzymes (alkaline phosphatase and anti-Gastropub acid phosphatase) activities in osteoblast cell, and increase the calcium contents. Theextract of Rehmmannia could also significantly inhibit the osteoblast G0/G1phasearrest induced by microgravity through the determination of flow cytometry, andrestore the normal cell metabolism functions.The security concentration ranges ofRehmannia-Gikgo biloba complexation were studied at the cellular level and animallevel. Through establishment of radiation microgravity model of mice, the in vivoradio and bone loss protective effect of Ginkgo biloba-pollen and Rehmanniacomplexation were systematically studied. Results indicated that the complexationcould effectively enhance the antioxidant enzymes (SOD, CAT, LDH and GSH-Px)activity in mice and reduce MDA levels. The results showed Rehmannia-Gikgobiloba complexation can be able to enhance the anti-oxidative system afterradiation.Studied the specific immunity and non-specific immunity,foundRehmannia-Gikgo biloba complexation can effectively increase the immune organindex,promote spleen lymphocyte and bone marrow stem cell proliferation andenhances monocyte chromosome aberrations and DNA content,result indicated thatRehmannia-Gikgo biloba complexation could protect the radiation induced DNAdamage. Results of bone loss protection indicated that the complexation couldeffectively protect the immune organs (thymus and adrenal gland) against radiationinduced damage and reduce the weight loss of dry matter in mouse bone. Theorganic matter, inorganic matter and bone metabolism related enzymes in mice bonesubjected to radiation and microgravity were studied, results indicated thecomplexation could significantly increase the organic matter and inorganic mineralsalts content in bone, as well as enhance the activity alkaline phosphatase enzyme inserum, which provided better protective effect on bone. This study possessed certaintheoretical value and practical significance to reveal the protection approach anddevelop double effect protection both radiation and microgravity.