Trichoderma Viride Screening And Combination Of Secondary Fermentation Of Straw

The aims of this study were to screen high-yield cellulase strains and to improve theproduct enzyme conditions. Based on seven trichoderma viride strains, seed medium andfermentation medium processing, the enzyme activity of the C₁, C_X, C_Band degradationrate of cellulose and lignin were assayed to screen good strains with higher enzymeactivity and degradation rates; By antagonism experiment of Trichoderma viride andwhite-rotfungi, two bacteria should be mixed fermentation for the step two todegradation of straw; Trichoderma viride and white-rot fungus mixed fermentationconditions was optimized by the proportion of straw and bran, the water content inmedium, total inoculum of mixed bacterias, inoculation ratio of mixed bacterias,respectively; Adopted two levels in vitro digestion test, the in vitro digestibility of thefermented straw was evaluated and the results were as follows:（1） CMC enzyme and filter paper enzyme activity from Trichoderma viride L4C andBS were the highest when straw as the main carbon source, and the activity of them was2438.4U/mL,2438.4U/mL,715.4U/mL and658.8U/mL, respectively. When wheatused as the main carbon source, CMC enzyme and filter paper enzyme activity fromtrichoderma viride L4C and BS became the highest, and the activity of which was2854.0U/mL,3237.7U/mL880.5U/mL and865.7U/mL, respectively.（2） The test to use mixed fermentation of Trichoderma viride and white-rot fungishowed that the fermentation time was six days, and the best combination was O6-H10,namely white-rot fungi inoculated for6days, then inoculated trichoderma viride for10days.（3） The optimization condition of Trichoderma viride L4C, BS and white-rot fungion straw medium were as follows: the proportion of straw and gluten for7:3,6:4, thewater content in medium for55%,45%, the total inoculum of mixed bacterias for15%,25%，the inoculation ratio of mixed bacterias all for3:2. Undering the bestfermentation conditions, the hemicellulose, cellulose, lignin degradation rate of mushroom and Trichoderma viride L4C were36.4%,41.6%and48.1%，respectively.namely that were1.5times,1.22times and2.5times, respectively. The degradation rateof bacteria fermentation; mushroom and Trichoderma viride BS were31.4%,31.5%and52.9%, respectively, namely that are1.3times,1.05times and2.75times, respectively.The optimization condition of Trichoderma viride BS, L4C and white-rot fungi on wheatmedium were as follows: the proportion of wheat and gluten was7:3, and the watercontent in medium was55%, the total inoculum of mixed bacterias was15%，and theinoculation ratio of mixed bacterias was3:2.（4） In vitro two levels digestion test showed that dry matter digestibility of mixedbacterias fermentation was reach to40%～60%, that was1.79～2.82times as high ascontrol.