The Biological Effects Of Cerium Oxide And Europium Doped NaYF4Nanoparticles On Endothelial Cells

Over the past decades, with the rapidly development of the nanotechnology more andmore nanomaterials were be prepared and widely used in industry, agriculture and biomedicalfields. However, with the applications of those nanomaterials constantly increases, the risk ofhuman exposure rapidly increase. So, the study of the biology effect research about thenanopaticles to cells has a great significance. Due to the low density and small diameter,nanoparticles can easily suspend in the air and through breathing into the biologyenvironment. Then, they will translocation across biological barriers and transported to theother organs by the blood vessel. In addition, when the nanoparticles were applied in thebiomedical field, they would be injected into the body via intravenously injection. All of thosewill lead to the vascular endothelial cells direct contact with these nanoparticles. Thus, it isnecessary to study the biology effect of nanoparticles to endothelial cells (ECs). In this study,we use the endothelial cells as a cell model to explore the biological effect of two kinds ofnanoparticles (nano cerium oxide and nano NaYF4:Eu3+), which have been widely applied.The main research results are as follows:1. Cerium oxide nanoparticles protect ECs from apoptosis induced by oxidative stressCerium oxide nanoparticles in the market as the research object, which werecharacterized by scanning electron microscope (SEM), X-ray diffractometer (XRD) anddynamic light scattering (DLS). Then set ECs as a cell model to study the antioxidantproperties of these nanoparticles on cells. The results indicating that the nanocaria’s diametersare about20nm and composed of uniform and pure nanoparticles. Those nanoparticles canenter into ECs were through caveolae-and clathrin-mediated endocytosis. And they caneliminate the exogenous H2O2and protect ECs from apoptosis effectively.2. The cytotoxicity of NaYF4:Eu3+nanoparticles in endothelial cellsThe NaYF4:Eu3+nanoparticles with three different size were used in this studies canenter into ECs and distributed throughout the cytoplasma of cell, but not specific targeting tomitochondrion or lysosome. The intracellular nanoparticles can decrease the cell viability,induce the intracellular Lactate dehydrogenase (LDH) release, arrest the cell cycles andinduce cells apoptosis through oxidative stress. All of the cytotoxicity exhibited by NaYF4was in a size-dependent manner.