Fabrication Of Targeted Porphyrin Gold Nanorod Nanocomposite Materials

The adoption of near infrared region imaging and therapy are also growing rapidlydue to the availability of gold nanorod. The wavelength range of650~1450in the regionof the spectrum with the lowest absorption.So hyperthermia wavelength in nearinfrared region enables the deepest tissue penetration, also it is special significant thelowest auto-fluorescence from tissue which is available to perform deep tissueimaging.NIR nano-composite received considerable highly efficient multifunctionalnano-composite material for targeted near infrared region imaging and hyperthermiatherapeutic strategy.Here, based on Gold Nanorod(GNR), Porphyrin (P), folate acid (FA), and colloidchemistry technique were used to design and construct a gold nanorod porphyrin-folicacid nano-composite (GNR-P-FA). GNR-P-FA has a covalently assembled monolayer oforganic–soluble folate acid-porphyrin compounds, resulting gold nanorod encapsuledby porphyrin on the entire surface. Transmission Electron Microscopy (TEM),UV-Visible Spectrophotometer and MOLDI-TOF/ESI Mass Spectral were employedto characterized the GNR, porphyrin and GNR-P-FA. Based on Localized SurfacePlasmon Resonance (SPR) of gold nanorod, the structural, optical properties andapplications in the diagnose and therapy were investigated.The Major Contributions are As Follows:1) UV-Visible spectrophotometer was used to compare the optical properties ofGNR with various aspect ratio. The results showed gold nanorod could wellUsing the TEM, a possible mechanism was proposed that Ag+optimize thestructure and optical proprieties.2) Gold nanorods (GNR-S) are using a place-exchange reaction functionalizedwith a thiol derivative. The changes in the Plasmon absorption bands indicatesignificant changes in the gold rod surface molecular, during theplace-exchange reaction, and have the organic solubility propriety.3) Five Porphyrin meso-chlorate porphyrin, four silica porphyrin compounds andfolate acid porphyrin were synthesized. And characterized by UV-Visibleabsorption,fluorescence spectrum, mass spectral.4) UV-Visible spectrophotometer for employed to characterize the GNR-P-FA,the decrement of Soret band absorption,showed that over80%energy wastransferred to gold nanorod, which can reduce the photo toxicity of imaging.Over100photosensitizing molecules were covalently bonded to every goldnanorod, On the basis of selective energy transfer from porphyrin to goldnanorod.GNR-P-FA exhibited optical proprieties, which are different from those corresponding traditional nano-materials. UV-Visible Spectrophotometerfor employed to characterize the GNR-P-FA, the decrement of Soret bandabsorption showed that over80%energy was transferred to gold nanorod,which can reduce the photo toxicity of imaging. Over100photosensitizingmolecules were covalently bonded to every gold nanorod, On the basis ofselective energy transfer from porphyrin to gold nanorod.So, GNR-P-FAexhibited optical proprieties, which are different from those correspondingtraditional nano-materials.5) Different from popular approaches based on electrostatic or hydrophobicinteractions, With strong covalent AU-S linkages between GNR andporphyrin-folate acid.So GNR-P-FA are very stable without aggregation ordecomposition, which significantly strengthened the linkage and reduced theprobability of leakage/desorption of the porphyrin. The surface of goldnanorod have a large number of porphyrin molecular,this enables imagingwith high sensitivity in gold nanorod core. GNR-P-FA have advantages inincreasing organic porphyrin’s circulation time. Compared to single porphyrinmolecule. So imaging has the high intensity.