The Effect Of Pyocyanine In The Process Of Petroleum Hydrocarbon Biodegradation By Pseudomonas Aeruginosa

There are many microorganisms can produce pyocyanine in nature. Pyocyanine isone kind of redox substances, which can generate strong oxidants in vivo. The roleof pyocyanin in the process of biodegradation of petroleum hydrocarbons have notbeen reported. Pseudomonas aeruginosa can degrade petroleum hydrocarbons rapidly, itcan also be high-yield pyocyanin, research pyocyanin role in the process of degradationof petroleum hydrocarbons in Pseudomonas aeruginosa has important significance.There are four Pseudomonas aeruginosa strains mentioned in this thesis, pyocyaninpositive bacteria NY3and NX, and the corresponding negative mutant NY3-1and NX-I.This thesis studied the characteristics of pyocyanin which can be Synthesis byphotochemical and biological methods. We attempt to explain participation ofpyocyanin in hydrocarbon degradation process. And then, we studied the differencesbetween pyocyanin positive and the corresponding negative mutant strain in the processof degradation of petroleum hydrocarbon. To description the secretion of pyocyanininfluent the ability of bacterial in degradation of petroleum hydrocarbons. The resultsare as follows:（1） Pyocyanine can be synthetized by pseudomonas aeruginosa and photochemicalreaction of Phenazine methosulfate, and resultant products share the same basicstructures.19.9%of hexadecane was removed after60minutes in the presence ofoxygen, ferrous ions and NADH. When pyocyanine, coenzyme NADH, phenanthrenecoexisted through reacted time of19h, intermediate products of the monohydroxy andtrihydroxyphenanthrene oxidation products were initially identified. （2） NY3metabolic efficiency of hydrocarbon production by NY3is higher thanthat of NY3-1. When Hexadecane is used as sole carbon source, the95h-positivebacteria biomass OD_600nmequals3.192, negative bacteria OD_600nmequals0.272,hexadecane degradation rate is24.8%higher than negative bacteria increased; whendiesel was used as a carbon source, positive bacteria growth OD_600nmequals3.228andnegative bacteria only1.094, a9.7%increase in the average degradation rate ofhydrocarbons in diesel synchronization than negative bacteria; as to degradationresistant crude oil, the growth rate of positive bacteria NY3is more fast that that ofnegative bacteria NY3-1and crude oil degradation rate increased by9.12%. Productionof pyocyanin positive bacteria NX and its corresponding negative mutant NX-Ishowed the same results, positive bacteria NX are20.8%higher than negative mutantstrains in the diesel fuel degradation. The wax alkanes increased by22.21%thanaverage degradation rate.（3） The pyocyanine NY3and NX and the corresponding negative mutant strainsNY3-1and NX-I produce acids during metabolic process. Acid-producing ratesincrease by bacteria in the process of growing metabolism. Positive bacteria acidsecretion rate obviously was significantly higher than the negative mutant.