Study On Technology Of Asparagus Juice And The Effect Of Metabolism Of Glucose And Lipid In High-fat Diet Induced Hyperlipidemic Mice

Asparagus officinalis L. is a kind of vegetable with high nutritional value and health care function. With the tender stems of asparagus used mostly at present, the old stems haven’t been used completely. In order to prevent resource waste, enhance the full utilization of asparagus and for deep exploitation, the processing technology and activity study on decreasing serum lipid was investigated; simultaneously a research on market investigation and feasible analysis on industrialization of AEO was made.The clarification of juice from asparagus with ZTC1+1-Ⅱ natural clarifying agent was investigated with the orthogonal design in the base of analyzing of single factor. The optimum of clarifying conditions was as follows:component B with8%was added for3hours at50℃, and then component A with4%was added for3hours at50℃. After that asparagus juice whose clarity was as high as98.9%contained much polysaccharide, saponin and so on. It was showed that the stable products could be obtained by simple manipulation. Asparagus extracts from old stems (AEO) was concentrated from clarified juice.Meanwhile, this research investigated the effect of asparagus extract on the glucose and lipid metabolism in high-fat diet (HFD)-induced hyperlipidemic mice. HFD mice models were induced by high-fat diet for4weeks, and AEO group mice were administered orally with AEO at dose of1g/kg,1.5g/kg and2g/kg per day for12weeks. The results showed that AEO could cause significant reduction in food intake and water intake; and the body weight decreased remarkably after administrated with1.5g/kg AEO. It was also appeared that AEO of low and medium dose led to significant decrease of serum cholesterol and increase of high density lipoprotein cholesterol (HDL-C). The decrease percentage of lipid was23.4%and27.1%for low and medium dose, respectively. It was found that AEO could reduced LDL-C, and enhanced the ratio of HDL-C and TC. It was also showed that long time HFD feeding could lead to the disorder of glucose metabolism in experiment groups. AEO could decrease the level of high glucose in model mice which caused by long time HFD feeding. The oral glucose tolerance test showed that AEO could reduce serum glucose too. It was showed that AEO could reduce the serum glucose after glucose loading in30minutes and60minutes which manifested by significant reduction of area under the curve of blood glucose, thus changed the abnormality of glucose tolerance. It was also showed that AEO could increase the insulin sensitivity index, but caused no significant difference in serum insulin content. It was indicated that the activities of superoxide dismutase (SOD) and glutathione peroxides (GSH-PX) in the mice liver increased significantly after administrated with low and medium dose of AEO. However, AEO had no significant effects on other liver biochemical indexes such as total cholesterol (TC), triglyceride (TG), total anti-oxidative capacity (T-AOC), and glutathione (GSH). Based on the results above, it was concluded that AEO had a positive effect on the regulation of glucose and lipid metabolism in HFD-induced hyperlipidemic mice.Due to the technical indicator and decreasing serum lipid of AEO from old stems, it is concluded that the industry will have prosperous future and economic efficiency through market investigation on industrialization of AEO.