| The emergence of new nanomaterials, accompanied by the development ofnanotechnology, new ideas about the design of efficient biosensors have been provided withnew vitality. Metal nanomaterials with excellent optical properties can be used as theidentification element of the biosensor, especially when it combined with the targets to outputthe signal with optical. So nanometal fluorescence materials open up a vast world for theconstruction of biosensor and attract a lot of attention in the analysis of life. In this thesis, thenano-metal fluorescence materials, such as copper nanoclusters, silver nanoclusters, rare earthupconversion luminescence, which as a platform of noval biosensor to fast, simple, green ofdetection physioloical and pathological regulation related biothiols compounds, AChE and theinhibitors of paraoxon, the environment-related pH, respectively.In Chapter2, biothiols interacte with double-strand DNA-templated synthesis of coppernanoclusters, which was found the fluorescence of copper nanoclusters quenched effectivelywith the increase of biothiols. Accorrding to the experiment phenomenon, we reported asimple, environmental and selective method to detect biothiols including Cys, GSH, Hcy. Theresulted calibration plots exhibited good linear correlations in the range from2.0×10-6to1.0×10-4mol/L for Cys,2.0×10-6to8.0×10-5mol/L for GSH, and5.0×10-6to2.0×10-4mol/Lfor Hcy, respectively. Meanwhile, the synthesis of copper nanoclusters was quite simple,which would fastly report to biothiols and have high selectivity. In addition, the method wassuccessfully applied to the detection of biothiols in human plasma samples.In Chapter3, because acetylcholinesterase （AChE） can catalytic thioacetyl choline （ATch）to generate thiocholine. According to the previous report, mercapto compound was able toeffectively quench the fluorescence of silver nanoclusters which was synthesised bysingle-stranded DNA as the template However, the generation of thiol-compounds dependedon the concentration of AChE, so the silver nanoclusters fluorescence quenching relied on theconcentration of AChE. A linear relationship was presented within a certain range, whichcould be used to detect AChE environmental-friendly, sensitively and simple-effectively.While, paraoxon could be furtherly detected since the catalytic action of AChE could beinhibited by the organic phosphorus. The good linear dynamic detection were revealed to be7.5×10-4to1.5×10-3U/mL for AChE,1.0×10-8to1.0×10-3μg/mL for paraoxon, respectively.In chapter4, As fluorescein isothiocyanate （FITC） could quench the fluorescence ofupconversion nanoparticles at475nm, FITC absorbance at475nm made a difference with thechange of pH. As a result, FITC quenched the fluorescence at475nm of UCNPs was relatedwith pH. Therefore, we constructed a fast, simple and sensitive method to detect pH withFITC-UCNPs. |
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