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Degradation And Application Of Aflatoxin B1by Aspergillus Niger

Posted on:2013-04-25Degree:MasterType:Thesis
Country:ChinaCandidate:B LiFull Text:PDF
GTID:2251330425478444Subject:The vet
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Aflatoxins are secondary metabolites produced by Aspergillus flavus and Aspergillums nomius. it’s a class of mycotoxin with the similar structure and physicochemicalproperty, and it is also a highly toxic and carcinogenic substances of which AFB1is the most stable and the most toxic mycotoxin that have discovered up to now. Aflatoxin is widely exist and is dangerous. If AF pollute animals’feed, it may reduce animals’production performance, and it may cause a series of diseases as well. The aflatoxin can be indirectly get into human body through food chain and has a strong carcinogenic effec that serious threaten human health. The paper did a research on aflatoxin Bl’s screening on degradation strains, the optimized condition on how aspergillus to degrate aflatoxin Bl, and the research and application of aspergillus degradation of aflatoxin. Our main results are as followed:(1) Aspergillus niger was screened out of the300strains, including a strain of lactic acid bacteria, a strain of Aspergillus oryzae and Aspergillus niger, the degradation ratio of AFB1reached93.28%.(2) The fermentation conditions of AFB1degradation was obtained as follows: inoculation amount5%, optimum temperature40℃, pHS.Oand150r/min for72h.(3) AFB1was effectively degraded by extracellular extracts from aspergillus niger, the degradation ratio reached84.20%. And it proved that aspergillus niger’s degradation effect on AFB1is like the effect of degrading enzymes(4) Aspergillus niger and its degrading enzymes do not cause the acute toxic effects, it has good effect on degradation to AFB1of moldy feed.The main innovation:(1) This test screening Aspergillus niger, the degradation ratio of AFB1reached93.28%.(2) This test proved that was A favorable safety of aspergillus niger, they can be applied to feed or food industry.
Keywords/Search Tags:aspergillus niger, aflatoxin B1, degradation, application, feed
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