Effect Of Dynamic High Pressure On Structures And Physiological Function Of Hemicellulose From Soybean Dregs

Hemicellulose is a very important of dietary fiber, but its complexity of their structuresand high molecular weight limited the exertion of its various property. The dynamic highpressure microfluidization (DHPM) is a new way of physical modification. The results haveshown that DHPM can make macromolecules fracture and decrease molecular weight, thenimproves the property of material.HCLB-1and HCLB-2isolated and purified from hemicellulose B (HCLB) were treatedby the dynamic high pressure microfluidization in the paper. The effect of DHPM on itsstructure and physiological functions were studied, providing a new method for modificationon hemicellulose and the theoretical basis for researching and developing the resource ofhecellulose B.The results of the paper were as following:(1) Hemicellulose B was isolated by caustic extraction-ethanol precipitation method. Theoptimal volume ratio was1:2; The purification procedure of HCLB polysaccharide wasestablished by using DEAE-Sepharose Fast Flow column and two main componentsHCLB-1、HCLB-2were obtained. The result of ultraviolet spectra and high performance gelpermeation chromatography (HPGPC) indecated there were no nucleic acid and protein inthem. In addition, the research indecated HCLB-1and HCLB-2were homogeneouspolysaccharide components. The results demonstrated that DEAE-Sepharose Fast Flowcolumn is compatible for the purification of hemicellulose polysaccharide and this method isfast, simple, effective and fit to be magnified.(2) The molecular weight of HCLB polysaccharide was tested by high performance gelpermeation chromatography (HPGPC). The molecular weights of HCLB-1and HCLB-2were3890451and2454708Dal.. The major monosaccharide in both HCLB-1and HCLB-2isxylose and arabinose by GC analysis. The primary structures of HCLB-1、HCLB-2were obtained by using methylation analysis methods and many instrumental methods (such as IR,13C-NMR, etc.). The structure of HCLB was that the (1�'4)-xylopyranose backbone wassubstituted on O-2and/or O-3by single residue or short chains.(3) After being treated by DHPM, the molecular weight of HCLB reduced. Some HCLBof big molecular weight were broken into small molecular substance; the characteristicabsorption peak of HCLB showed blue shift or red shift in different degree after it was treatedby DHPM; the filamentous structure which had one shape of ‘match heads’ of HCLB had onechange of the long filament cutting and ‘match heads’ ablating.(4) After being dealed with DHPM, HCLB had the apparent proliferative effect onbacillus bifidus and lactic acid bacteria, and it had the inhibition effect on escherichia coli.The results showed HCLB had the effect of adjusting intestinal flora.