Preparation And Application Of Cellulose Ester Coated Chiral Stationary Phases With Different Silica Supports

Several cellulose tris(3,5-dimethylphenylcarbamate)(CDMPC) coated chiral stationaryphases (CSPs) with different supports were prepared and evaluated in this paper.Enantioseparation of twelve netural or acidic compounds were evaluated. The aim of thisstudy is to develop new chiral columns to widen the enantioseparation needs of different typesof chiral compounds.This paper mainly covers the following aspects.1. Cellulose tris(3,5-dimethylphenylcarbamate) was prepared by the reaction ofcellulose and3,5-dimethylphenyl isocyanate. The product was coated on the silica gel,aminopropylsilylated silica gel and mesoporous silica SBA-15, respectively. The obtainedCSPs were denoted as SG@CDMPC, APS@CDMPC and SBA-15@CDMPC.Enantioseparation of twelve neutral or acidic chiral compounds were performed on thesehomemade CSPs in the normal phase mode. The results were compared with theenantioseparation on the commercial CSP, Chiralcel OD-H. Six compounds obtained bettercolumn efficiency on SG@CDMPC than on Chiralcel OD-H, and three of them obtainedbetter enantioseparation. The enantioseparation ability was poorer on APS@CDMPC andSBA-15@CDMPC than on SG@CDMPC and the commercial one on the whole.2. Enantioseparation of warfarin was investigated on SG@CDMPC and APS@CDMPCin the normal phase mode. The effects of column temperature and alcohol modifiers in themobile phase were investigated. Besides, the mechanism of chiral recognition of the CSPswas discussed by analyzing the thermodynamic parameters of enantioseparation of warfarin.