Study On The Nitrification Of Low Temperature Nitrifying Bacteria In The Sewage

Two kinds of low temperature nitrifying bacterias are cultivated by solid medium and liquid medium.The purified bacterias are added to the activated sludge and the constructed wetland. We observe the nitrification of the bacterias in the domestic wastewater and use the microscopic to see the biological phase in the sets. The bacterias are identified by Gram stain, plate counts, BIOLOG and PCR identification.The results indicate that the ammonia nitrogen removal rate of the adding self-made bacteria system is increased to60%, and its COD removal rate is increased to85%. These two indicators are higher than the sludge system without self-made bacterias. In the quick start, the self-made bacterias can start in the fifth day. The sludge with self-made bacterias are three days earlier than the sludge without the strains. And the self-made bacterias are better than the BE bacteria which is purchased. The ammonia nitrogen removal rate of the adding self-made bacteria wetland can stabilize at40%, and it is10%-15%higher than the wetland without the self-made bacterias. The bacterias have effect on the total nitrogen and COD at the same time. The experiments show that the self-made bacterias have the nitrification ability and the synergy with the organic heterotrophic bacterias. According to a series of means, these two bacterias are milk white, smooth, moist, intermediate uplift, non-nuclear, opaque, neat edges and Gram-negative bacteria. The concentration of the X strain in the liquid medium is7.5×104per mL, the Y bacteria is6.7×104per mL. The BIOLOG and PCR identificate that the X bacteria is Pseudomonas Mendoza and the Y bacteria is Enterobacter cloacae.