| Anilofos herbicides is used on a large scale all over the world. With the increasingly serious heribicides residues, the anilofos residual problem is also valued. To date, no ELISA for the specific determination of anilofos has been reported. Therefore, developing rapid, sensitive methods to detect anilofos residues is of great importance. In our study, a highly sensitive and specific ELISA was developed for detecting anilofos residues and was successfully used for the rapid analysis of agricultural and environmental samples.Hapten was artificially produced according to the structure of the anilofos. The anti-anilofos polyclonal antiserum with a titer of1:160000was obtained which had an inhibition of59.76%to anilofos (100μg/L). High purity antibodies were obtained after the antiserum purified by Protein A-Sepharose4B. ELISA methond was established by the optimization of varieties conditions. The limit of detection (LOD) was0.1±0.009μg/L, the sensitivity was1±0.05μg/L, and there was no cross-reactivity with other related pesticides or structurally similar compounds. The inter-assay and intra-assay were lower than15%.Matrix interference was removed easily by exacting and diluting. Recovery of anilofos ranged from81%-116%with a coefficient of variation (CV) of1.7%-9%.Samples were validated using GC and the results showed a good correlation with the dc-ELISA data (R2=0.9795). Therefore, the developed immunoassay is suitable for the rapid quantitative or qualitative determination of anilofos residues in agricultural products and environmental samples.42random samples collected from different supermarkets were analysed using the developed dc-ELISA, no anilofos was found in the commercialized cereal products. The developed immunoassay was verified to be suitable for the rapid quantitation of anilofos residues in practical samples. |
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