| With the fast development of modern industry and agriculture, antibiotic pollution is becoming more and more serious. Antibiotic pollution could cause persistent toxic effects to environment as it could not be turned into non-toxic form via chemical or biological means in natural condition. What’s worse is some antibiotic could be transformed into organic compounds that have stronger toxicity. The antibiotic in the environment could get access to human body through food chain, thus causing damaging harm to environment and human health.For most of the traditional detection methods, large equipment is usually need, the cost is high and the samples must be digested, only one kind of antibiotic could be detected at one time, process of multiple antibiotics detection is complex, requirement of on-time and in situ detection could not be met, thus is of great importance to develop new and fast detection method.(1) A label-free, aptamer-based sensor system for the detection of tetracycline (TET) in aqueous solution was designed. Results show that in the optimized condition,250μL AuNPs,100μM NaCl,190μL MOPS buffer (10mM, pH7.0),200～1000nM of TET could be detected linearly with60nM TET-aptamer, with lowest detection limits (LOD)17.6nM in colorimetric and24.2nM in resonance light scattering. The sensor system shows good selectivity.(2) The selected sequences that has high affinity with kanamycin (KANA) was obtained. These intact sequences were picked out to carry out further study, application in KANA detection by colorimetric. Results show that all of these sequences could bind with KANA selectively. These sequences were applied in AuNPs-aptamer based KANA sensor system, it reveals that in the optimized condition,100μL gold nanoparticles (AuNPs),10mM MOPS phthalic diglycol diacrylate (PDDA, diluted50000times),375μL3-(N-morpholino) propanesulfonic acid (MOPS) buffer (10mM, pH7.0),2nM of these sequences could detect KANA linearly in different range, with a lowest detection limit (LOD)15.75nM. |
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