Study On The Detection Methods Of UV Filtes And Heavy Elements Of Lead And Arsenic In Cosmetics

This thesis focused on analysis methods studies for determination of UV filters allowed and heavy metals restricted in cosmetic products. The mainly studies were summarized as follows:1. A reversed phase high performance liquid chromatography method was established for determination of thirteen UV filters in cosmetics, including p-Aminobenzoic acid esters and their derivatives, Cinnamic acid esters, Salicylic acid esters and their derivatives, Benzophenone ketones, Camphor derivatives. The UV filters in cosmetics were extracted by methanol, then were separated on Agilent Eclipse Plus C18column (250mmx4.6mm, i.d.5μm) with35Celsius in gradient elution mode using methanol and0.1%(v/v) phosphate acid as mobile phases. The results of the experiments showed that the linear correlation coefficients of thirteen UV filters were larger than0.9995, and the detection limits varied from0.15to1.2mg/L. The relative standard deviations ranged from0.04%to3.6%, and the spiked recoveries of real cosmetic products were in the range of72.5%to110.6%.2. A method for determination of Drometrizole trisiloxane, Ethylhexyl triazone, Disodium Phenyl dibenzimidazole tetrasulfonate, Bis-ethylhexyloxyphenol methoxyphenyl triazine in cosmetics by high performance liquid chromatography wad developed. Acetone was used to extract these UV filters from cosmetics. The mobile phases were methanol and water phases with gradient elution mode and31Inm was selected as detection wavelength. Agilent Eclipse XDB-CN column (250mmx4.6mm, i.d.5μm) with35Celsius can separated these four UV filters. The results of experiments showed that the linear correlation coefficients of four UV filters were larger than0.9995, and the detection limits varied form0.05to O.lmg/L. The relative standard deviations ranged from0.2%to1.1%, and the spiked recoveries of real cosmetic products were in the range of95.3%to108.6%.3. The microwave digestion-Graphite furnace atomic absorption spectrometry method was established for determination of harmful heavy elements of Leads and Arsenic in cosmetics. The cosmetic samples were digested in microwave digestion instrument using nitric acid and hydrogen peroxide mixed solution (8:1v/v) as digestion solution. In order to reduce matrix effects and improve the sensitivity of the methods, the phosphoric acid and palladium solution were added respectively as matrix modifier of heavy metals Lead and Arsenic to raise ashing and atomization temperaturethe to600℃,1200℃for Lead and1400℃,2600℃for Arsenic. The experimental results showed that the linear correlation coefficients of Lead and Arsenic were greater than0.9995, the detection limits were2.5μg/L and10μg/L for Lead and Arsenic respectively, the spiked recoveries of Lead and Arsenic were97%and102.3%.