Treatment Of Diosgenin Wastewater And Producing Glutathione And S-adenosyl-L-methionine By Fermentation Using The Wastewater

Diosgenin was the basic material in steroid hormone drug synthesis, The core problem of producing Diosgenin by using traditional acidolysis technology was that there were plenty of wastewater which was a kind of great acidity, thick color, high concentration organic and rich in carbohydrates, the pollution problem of wastewater which had such characteristics as high COD had been a hard nut to crack of the healthy development of the industry.In response to the general character outstanding problem of this industry, the viewpoint was proposed after pretreating with the wastewater and then fermented Glutathione and S-adenosyl-L-methionine by S. cerevisiae using the carbohydrates of Diosgenin wastewater. The way of utilization of wastewater comprehensively reduced environmental pollution.The Saccharomaces cerevisiae strain of high production of glutathione and S-adenosyl-L-methionine were screened by taking Glutathione productivity and S-adenosyl-L-methionine productivity as index by liquidstate fermentation in the study; By experiments, the fermentation conditions of strain were optimized; In the study, using the Diosgenin wastewater to produce Glutathione and S-adenosyl-L-methionine by fermentation, along with experiments to prove it woked, The influence factors and productivity of Glutathione and S-adenosyl-L-methionine which were produced through fermenting Diosgenin wastewater which were treated by three simple processing methods were discussed in this experiment. The COD removal rate of Diosgenin wastewater had been discussed, and providing a new approach for comprehensive utilization of Diosgenin wastewater.The strains of high production of glutathione and S-adenosyl-L-methionine were screened from24stains of Saccharomaces cerevisiae which were preserved in laboratory, the number was F2103.The fermentation conditions were mainly optimized through Single Factor design-Plackett-Burnman design-Response Surface Analysis.Finally, the optimal fermentation conditions of S-adenosyl-L-methionine were:inoculum volume10%and concentration of L-methionin4g/1and cultivated at30℃for56h and the initial pH6.0and60mL medium and the yeasts age24h and the agitation speed was180r/min. The predicted production was288.277mg/g on the model. Under the optimum fermatation conditions, S-adenosyl-L-methionine productivity was287.616mg/g, which was close to the prediction model and was1.38times of the initial. The optimal fermentation conditions of glutathione were:inoculum volume10%and concentration of L-methionin1g/1and cultivated at27℃for46h and the initial pH7.0and50mL medium and the yeasts age22h and the agitation speed was180r/min. The addition of L-methionine had no significant effect in Glutathione production through Plackett-Burman design, so L-methionin must be not added. The predicted production was20.986mg/g on the model. Under the optimum fermatation conditions, Glutathione productivity was21.039mg/g, which was close to the prediction model and was1.77times of the initial.The Diosgenin wastewater that produced by traditional process technology and pretreated with the wastewater were analyzed in three kinds of ways, The result indicated that different pretreatment embodied various effects. Considering the cost in the production and the production of Glutathione, Choosing the way of adjusting pH first and then absorbed with activated carbon. The fermentation yield of Glutathione reached0.836g/L, accounting55.02%of using pure sugar fermentation medium and removal rate of COD was77.60%; Likewise, Choosing the way of absorbing with activated carbon first and then adjusted pH. The fermentation yield of S-adenosyl-L-menthionine reached10.211g/L, accounting16.30%of using pure sugar fermentation medium and removal rate of COD was65.74%.The results signified that the process technology of using Diosgenin wastewater to produce Glutathione and S-adenosyl-L-methionine by fermentation is available. By means of comprehensive utilization of Diosgenin wastewater, and solve the problem of high pollution and energy consumption, low availability of resources and production efficiencies in the current Diosgenin industry. So it ensured the economy benefit of Diosgenin factories and provided a new approach for Diosgenin wastewater treatment. The great deal of primary experimental data and corresponding parameters in this paper can be referred for further study of Diosgenin wastewater treatment.