Study On The Characterization And Pathway Of Chloro Phenols Degradation By Fusariun Sp. HJ01

Chlorophenols (CPs) have been widely used in many industries. These compounds have high toxicity, persistence and effects of eco-environmental. Compared with physical and chemical method, biological method is cost-effective and easy-operated. In addition, CPs can be completely mineralized by microbes and none secondary pollutants produced. Now, the bacterial degradation of CPs has been intensively conducted. However, the degrading ability to refractory organic compound and adaptability to high toxicity conditions of fungi are higher than those of bacteria, which shows the advantages in environmental restoration. The purpose of this dissertation is to investigate the characterization of an isolated Fusarium sp. HJ01capable of degrading4-CP and2,4,6-TCP. This article focused on the4-CP degradation effect, degradation kinetics and degradation pathway by Fusarium sp. HJ01with co-existence of heavy metals. In addition, this article explores the characteristics, kinetics and pathway of2,4,6-TCP degraded by Fusarium sp. HJ01. The main conclusions of this research were as follows: (1) The characteristics and kinetics of4-CP degraded by Fusarium sp. HJ01with co-existence of heavy metals. The results show that0.15mM Pb2+and0.3～0.7mM Cd2+could accelerated the biodegradation rate of4-CP, while1～7mM Cu2+had suppressive effects on4-CP degradation.4-CP degraded by HJ01could be expressed in zero-order kinetics, and it would meet with Andrews model by adding extra carbon source.(2) The4-CP degradation pathway by Fusarium sp. HJ01with co-existence of heavy metals. The CC12O activity could be detected with co-existence of heavy metals, and the optimum reaction conditions were:40℃, pH=8. The concentration of heavy metals also affected activities, the results show that0.15mM Pb2+could promoted CC12O activities, while1～7mM Cu2+and0.3～0.7mM Cd2+decreased CC12O activities. Then GC-MS analysis results reported that4-chlorocatechol was an intermediate, and it can be identified that4-CP was biodegraded via catechol pathway by Fusarium sp. HJ01。(3)2,4,6-TCP degradation characteristics and kinetics of Fusarium sp. HJ01. It can be concluded that Fusarium sp. HJ01can use2,4,6-TCP as sole carbon source and energy source to grow. The optimal conditions for2,4,6-TCP biodegradation were:30℃, pH=6and the suitable nitrogen source was NaNO3. Under the condition of30℃and pH6, Zero-order kinetics accorded with2,4,6-TCP biodegradation well. While Halane-Andrews would accord with2,4,6-TCP biodegradation better by adding sugar or glucose as the extra carbon source.(4)2,4,6-TCP degradation pathway of Fusarium sp. HJ01. The results analyzed by GC-MS show that2,6-dichlorophenol was the intermediate, and2,4,6-TCP was degraded via2,6-dichlorophenol pathway by Fusarium sp. HJ01.