Research On Chemical Constituents And Biological Of Bacillus Marinus GS-5

Bacillus marinus was a species of Bacillus, some unique compounds were isolated from it due to its special environment and the studies of secondary metabolites from Bacillus marinus became popular in the world in recent years. China was a country of vast maritime territory and rich marine microorganisms, made full use of the advantage resources of our country’s marine microorganisms and made the research on natural active substances from marine microorganisms could find a lot of beneficial microbial active secondary metabolites for human beings.This paper chose the GS-5strain from marine sediments samples as a research object after the biological activity test and the active component identification of physicochemical properties. Based on PCR sequence analysis of the16S rDNA gene and neighbor-joining tree construction by blasting in GenBank indicated that the GS-5strain belonged to Bacillus marinus.Compounds extracted with ethyl acetate from the fermentation broth of the GS-5strain were separated by thin preparation layer chromatography, silica chromatography as well as high-performance liquid chromatography. Finally,14metabolites of the GS-5strain were obtained. The chemical structures were confirmed by physicochemical properties, NMR and MS techniques. They were identified to be:cholest-4-en-3-one (1). Diisobutyl phthalate (2).7-(-D-Glucopy-ranosyloxy)-3-(4-hydroxyphenyl)-4H-1-benzopyran-4-one(3). Cyclo(Va-1-Pro)(4). N-phenethylisovaleramide (5).3-Phenylpropionic acid (6). Cy-clo(Leu-hydroxypro)(7). Cyclo-(Phe-hydroxypro)(8).4’,7-dihy-droxyis-flavone (9)、Cyclo-(Leu-Pro)(10). Cyclo(Phe-Pro)(11).2-Phenylethylamine (12). Cyclo-(Tyr-Pro)(13).4’,5,7-trihydroxyisfl-avone (14)。Bioassay results indicated that four cyclodipeptides—compound4,7,8and11were capable of playing an inhibitory effect against the growth of Esherichia coli.In summary, we demonstrated in the present study that fourteen compounds were successfully isolated from GS-5strain fermentation broth and bioassay results indicated that compound4,7,8and11were capable of playing an inhibitory effect against the growth of Esherichia coli. The present study will provide a biological and chemical basis for generating the new antibiotics-like bioactive drugs from the secondary metabolites from Bacillus marinus.