The Research For The Fermentation And Application Of Bacterial Cellulose

Bacterial cellulose (BC), a kind of nano-biomateria with ultra-high puritl, isproduced by certain microbial strains. It is of particular network structure composed ofultra-fine ribbon-shaped fibers. In comparison with plant cellusole, BC displays highpurity, high crystallinity, high water-holding capacity, biocompatibility, highmechanical strength and controllable during the process of biosynthesis and so on.Therefore, BC can be used in areas where plant cellulose can hardly be used.Nowadays BC has been a hot one that attracted lots of scholars’ attention in thematerials field. At present, there are some problems about production and applicationof BC in China. With as Acetobacter xylinumM12a strain, study on the staticfermentation conditions based on bacterial cellulose (BC) was conducted. And basedon bacterial cellulose is as potential applications of immobilized material, theinfluences of fermentation conditions and drying methods on the porosity and thecapacity of holding water was discussed. Finally, a pilot study was to explore theviability of BC prepared as support materials was studied to prepare the bacterialcellulose. Main results for this study are as follows:1) The outcome indicated that the suited condition of M12was6%of inoculum size,36h of seed age, fermentation temperature30℃, optimum initial pH4.0~7.0andculture time6days.2) Using SEM and AFM to observe the morphology of cellulose membrane. And theperformance of the bacterial cellulose was studied. The method of drying had littleimpact on the moisture content of bacterial cellulose membrane. But it is veryimportant to control the drying conditions. In addition, with the increasing ofinoculation quantity and fermentation period, the porosity of bacterial cellulosemembrane was decreased.3) This article selects Lactobacillus plantarum as candidate strain, and through someexperiments had preliminary understanding on it. Research results show that theimmobilized bacteria had well tolerance for acid and bile salts than the free strain.Therefore, the bacterial cellulose had played an important role in the protection ofprobiotics.