Screening Of Degradation Microbial Of Marine Oil Pollution And Research Of Degradation Conditions

Development of oil industry caused sea pollution in varying degrees. Accumulation, transformation or reaction of petroleum hydrocarbons in the environment harm marine life directly.Petroleum hydrocarbons enriched in plants animals and human body through the food chain will further expand the hazards,so petroleum hydrocarbons have become key pollutions. Compared with the chemical, pHysical methods, bioremediation is regarded as the most promising means of environmental governance in the advantages of small investment and no secondary pollution,it is the most important way to completely eliminate oil pollution. Since the inverse sea water environment and the characteristic of alkane hydrophobic, the molecules were difficult to be attacked by microorganism, so that the degradation rate declined, the long-standing alkane in contaminated environment caused persistent contamination. Therefore, this paper was trying to improve alkane degradation in seawater by optimizeing the degradation and emulsifiers production condition of screened alkane degrading bacteria and strains mixing.This paper filtered an alkane degradated bacteria from the near coast of Dalian Bay by methods of16SrDNA;Optimized the alkane degradation condition of this strain and moderately halophilic bacteria H05by oscillation experiments, and investigated the ability of alkanes’utilization and degradation of H05, H34alone;Farther,test the ability to produce emulsifiers of the two strains, and conditions were optimized to produce emulsifiers;investigated the ability of alkanes’utilization and degradation of mixed strainsAlkane degradated bacteria from the near coast of Dalian BayJdentificated it as Alcanivorax sp.,named Alcanivorax sp. H34.Optimum growth temperature was30℃,Alcanivorax sp H34optimum growth of the initial pH was7, H05was8; H34and H05were optimized to give the best nitrogen source concentration of3g/1and4g/1respectively, the total concentration of the phosphorus source were3g/1; Under optimized conditions, paraffinic components average degradation rates of H34,H05growth cells were62.14%、76.29%, total degradation rate were47%and72.1%.Under optimized conditions, paraffinic components average degradation rates of H34,H05ungrowth cells were41.55%、45.65%, total degradation rate were45.49%、48.39%repectively.;The best medium for H05and H34to produce emulsifier was2216E medium with glucose as carbon source;the optimal carbon concentrations were7%,5%repectively, nitrogen ammonium concentration was1%.Mixed strains component average degradation rate was82%, compared with H05and H34, increased by7.5%,32%respectively, the total degradation rate was79.51%, compared with H05and H34increased by10.3%and40.8%respectively. mixed strains’ component average degradation rate was56.72%, compared with H05and H34increased by24.25%and36.51%, the total degradation rate was60.18%, compared with H05and H34increased by24.36%and32.29%.