Study On Extraction Of Antibiotics And Pigment By Aqueous Two-phase System

The aqueous two-phase system (ATPS) was applied for extracting antibiotics in theblood and pigment in foods. A new method ATPS was established. Using acetonitrile/salt aqueous two-phase system to extract antibiotics in blood, ionic liquid/anionicsurfactant two-phase system sudan in candy and detected by high-performance liquidchromatography (HPLC).Acetonitrile/salt aqueous two-phase system was developed to extract thesulfonamides from blood.3.00mL of blood sample was diluted with1.00mL puritywater and6.00mL of acetonitrile and1.0g of K2HPO4were added into the sample.The mixture was ultrasonically shaken for5min. The ATPS was formed. Thehemoglobin in blood was gathered together and suspended between acetonitrile andaqueous phases. The optimum conditions for the extraction solvent is acetonitrile andthe volume is6mL, the salt selected as K2HPO4and the quality is1.0g, extractiontime was selected as5min, pH was5. The limits of detection forsulfamonomethoxine, sulfachlorpyridazine, sulfamethoxazole and sulfafurazole were4.81,1.87,2.73,2.17μg L-1, respectively. When the present method was applied tothe analysis of blood samples, the recoveries of the analytes ranged from80.1to96.9%and relative standard deviations were lower than7.13%.The ionic liquid/anionic surfactant aqueous two-phase system was developed and applied for the extraction of Sudan Ⅰ-Ⅳ from candy. High-performance liquidchromatography (HPLC) were applied to determine the analytes. The aqueoustwo-phase system (ATPS) was formed in the present of C4[MIM]BF4,sodium dodecylbenzene sulfonate and (NH4)2SO4. The parameters affecting the extraction efficiency,such as volume of ionic liquid, amount of sodium dodecyl benzene sulfonate, ionicstrength, pH value of system, extraction time and temperature were investigated. Thelimits of detection for Sudan Ⅰ， Sudan Ⅱ， Sudan Ⅲ and Sudan Ⅳ were5.45,4.66,3.68,4.20μg Kg-1, respectively. When the present method was applied to theanalysis of candy samples, the recoveries of the analytes ranged from82.3to112.1%and relative standard deviations were lower than7.41%.